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利用日本岛津RF-5301PC荧光分光光度计,对枸杞子粉末中的维生素B2(VB2)直接进行荧光光谱检测,采集的光谱范围在530～650nm波段。采用MATLAB数值微分和高斯函数拟合方法,得到在激发波长为480nm、峰值波长为563nm时的荧光光谱信息;通过数值积分和线性拟合,得到荧光强度沿波长的积分与VB2含量呈良好的线性关系,其相关系数为0.998 43。实验测定枸杞粉末VB2含量为1.28±0.17μg/g,并阐释了枸杞子粉末荧光光谱红移的原因。研究表明:这种固体粉末荧光光谱检测方法具有快速、直接、简单和环保等优点,对药品成份检测、食品安全监测等领域的应用具有重要的参考价值。
Using Shimadzu RF-5301PC fluorescence spectrophotometer, the vitamin B2 (VB2) in Goji berry powder was directly detected by fluorescence spectroscopy. The spectral range was 530-650 nm. Fluorescence spectra were obtained at 480nm excitation wavelength and 563nm peak wavelength using MATLAB numerical differentiation and Gaussian function fitting method. The integral of fluorescence intensity and the linearity fit of VB2 showed good linearity The correlation coefficient was 0.998 43. The content of VB2 in Lycium barbarum powder was determined to be 1.28 ± 0.17μg / g by experiment, and the reason for the red shift of the fluorescence spectrum of Fructus Lycii was explained. The results show that this solid-state fluorescence spectrometry has many advantages, such as rapid, direct, simple and environmental protection. It has important reference value for the application of pharmaceutical ingredients testing and food safety monitoring.