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观察子宫内膜癌组织中内分泌分化细胞的增殖与凋亡状况、雌激素受体 (ER)与孕激素受体 (PR)表达 ,探讨其生物学及临床意义。手术切除或活检的子宫内膜癌标本 5 0例。采用嗜铬素A(CgA)作为内分泌分化的标记 ,进行CgA PCNA及CgA TUNEL双重染色 ,观察内分泌分化细胞的增殖与凋亡状况 ;并进行CgA与生存素 (Survivin)双重免疫组化染色 ,探讨生存素与内分泌分化细胞凋亡的关系。通过CgA ER及CgA PR双重免疫组化染色观察内分泌分化细胞的ER、PR表达。结果显示CgA阳性细胞呈PCNA及TUNEL染色阴性。CgA阳性细胞多表达Survivin。CgA阳性细胞密集的区域ER或PR减少明显。大多数CgA阳性细胞不表达ER或PR。结果提示子宫内膜癌组织的内分泌分化细胞属暂不增殖细胞群 ,亦极少发生凋亡 ,是较稳定的细胞群。内分泌分化细胞高表达Survivin可能是其逃避凋亡的分子学基础。子宫内膜癌组织的内分泌分化与ER和PR的减少有关
To observe the proliferation and apoptosis of endocrine differentiated cells in endometrial carcinoma, the expression of estrogen receptor (ER) and progesterone receptor (PR), and to explore its biological and clinical significance. Surgical resection or biopsy specimens of endometrial cancer 50 cases. CgA was used as marker for endocrine differentiation. Double staining of CgA PCNA and CgA TUNEL was used to observe the proliferation and apoptosis of endocrine differentiated cells. Double immunohistochemical staining of CgA and Survivin was also performed The Relationship between Survivin and Endocrine Differentiation and Apoptosis. The expression of ER and PR in endocrine differentiated cells was observed by CgA ER and CgA PR double immunohistochemical staining. The results showed that CgA positive cells were negative for PCNA and TUNEL staining. CgA positive cells overexpress Survivin. ER or PR decreased significantly in CgA-positive cell-dense regions. Most CgA positive cells do not express ER or PR. The results suggest that the endocrine differentiation of endometrial cancer cells are not proliferating cells, also rarely occur apoptosis, is a more stable cell population. The high expression of Survivin in endocrine differentiated cells may be its molecular basis for escaping apoptosis. Endocrine cancer endocrine differentiation ER and PR reduction related