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目的:探讨未成熟卵母细胞体外培养(IVM)在体外受精(IVF)中对卵巢低反应性者的补救性治疗效果。方法:30例行IVF时表现为卵巢低反应者(实验组)在B超引导下经阴道行卵泡穿刺术,取卵后对不同期未成熟卵母细胞行IVM,再行胞浆内单精子注射(ICSI)受精;对成熟卵母细胞则进行常规受精,胚胎形成后行移植术。取同期进行ICSI周期治疗的63例作为对照组。结果:应用IVM行IVF-ET补救治疗的30个周期中有29个周期获胚胎移植,临床妊娠9例(31%),胚胎种植率18.47%,但该组未成熟卵培养成熟后的受精率、卵裂率均低于对照组;总妊娠率及胚胎种植率比常规ICSI组低。结论:IVM技术联合IVF为卵巢低反应性者提供了一种有效的补救措施,明显减少了IVF周期的取消率,提高了妊娠机会。
Objective: To investigate the curative effect of immature oocyte culture in vitro (IVM) on ovarian hyporeactivity in in vitro fertilization (IVF). Methods: 30 patients underwent IVF showed low ovarian response (experimental group) under the guidance of B-transvaginal follicular puncture, ovulation IVIM at different stages of immature oocytes, and then the cytoplasmic sperm Injecting (ICSI) is fertilized; mature oocytes are routinely fertilized and implanted after embryo transfer. Sixty-three ICSI patients treated at the same period were selected as control group. RESULTS: There were 29 cycles of IVF-ET remediation in 29 cycles of embryo transfer, 9 cases of clinical pregnancy (31%) and 18.47% of embryo implantation rate. However, the immature egg fertilization rate , Cleavage rate were lower than the control group; total pregnancy rate and embryo implantation rate lower than the conventional ICSI group. CONCLUSION: IVM combined with IVF provides an effective remedy for ovarian hyporeactivity, significantly reducing the IVF cycle cancellation rate and improving pregnancy chances.