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目的 探讨 α-二氢麦角隐亭 DHEC对脊髓损伤后由血管痉挛造成 2次神经损伤的保护作用。 方法 对 72只 Wistar大鼠行阻断腹主动脉 2 5 m in后再灌流 ,制备成缺血性脊髓损伤模型。大鼠分成 :α-二氢麦角隐亭、海得静、尼莫地平和盐水对照组 ,分别于药物治疗的 3d、7d、14d处死动物 ,进行 CNTF抗体的 ABC免疫组织化学染色反应。 结果 脊髓横切片的 CNTF免疫反应结果经图像分析和 SAS软件处理后表明 ,在综合对比中 ,3种药物与对照组相比都具有显著性差异 (P=0 .0 0 0 1) ;α-二氢麦角隐亭组与海得静组和尼莫地平组的比较也都具有显著性差异 (P=0 .0 0 0 1) ;而海得静组与尼莫地平组间的比较的差异无显著性 (P=0 .2 6 31)。 结论 α-二氢麦角隐亭、海得静、尼莫地平都有增强脊髓损伤后脊髓运动神经元的 CNTF免疫反应性 ,而 α-二氢麦角隐亭的作用又强于海得静和尼莫地平。
Objective To investigate the protective effect of α-dihydroergocryptine DHEC on nerve injury induced by vasospasm after spinal cord injury. Methods Seventy two Wistar rats were subjected to occlusion of the abdominal aorta for 25 minutes and then perfused to prepare a model of ischemic spinal cord injury. The rats were divided into two groups: α-dihydroergocryptine, dextromethorphan, nimodipine and saline control group. The animals were sacrificed at 3d, 7d and 14d after drug treatment, respectively. ABC immunohistochemical staining of CNTF was performed. Results The results of CNTF immunoreaction of spinal cord transection showed that there was significant difference (P = 0.0101) between the three drugs and the control group in the comprehensive comparison after image analysis and SAS software processing; There was also a significant difference between the two groups (P = 0.00001), and the difference between the two groups No significant (P = .2 6 31). Conclusions Both α-dihydroergocryptine, dextromethorphan and nimodipine can enhance the CNTF immunoreactivity of spinal motoneurons after spinal cord injury, and the effect of α-dihydroergocryptine is stronger than that of holocervix and nimotone Horizon.