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目的通过检测B淋巴细胞诱导成熟蛋白1(Blimp-1)和B细胞淋巴瘤分子6(Bcl-6)在不明原因复发性流产(URSA)患者蜕膜组织中的表达水平,探讨两因子与URSA的关系。方法收集URSA患者(URSA组)和正常怀孕者(对照组)的蜕膜组织,采用实时定量PCR法检测蜕膜组织Blimp-1和Bcl-6的mRNA水平,免疫组织化学技术检测蜕膜组织Blimp-1和Bcl-6的蛋白水平,采用Pearson相关分析Blimp-1和Bcl-6的相关性。结果与对照组相比,URSA组蜕膜组织中Blimp-1和Bcl-6的mRNA均增加,Blimp-1的蛋白水平也显著增加,而Bcl-6蛋白表达水平增加不明显。Blimp-1和Bcl-6的mRNA表达水平显著正相关。结论 URSA组患者蜕膜组织中Blimp-1和Bcl-6表达增强。
Objective To detect the expression of Bcl-6 and Bcl-6 in decidual tissue of patients with unexplained recurrent spontaneous abortion (URSA) by detecting the expression of Bcl-2 and URSA Relationship. Methods The decidual tissues of URSA patients and normal pregnant women (control group) were collected. The mRNA levels of Blimp-1 and Bcl-6 in deciduas were detected by real-time quantitative PCR. The expression of Bcl- -1 and Bcl-6 protein levels, Pearson correlation analysis Blimp-1 and Bcl-6 correlation. Results Compared with the control group, the mRNA levels of Blimp-1 and Bcl-6 in decidual tissue of URSA group were increased, the protein level of Blimp-1 was significantly increased, but the expression of Bcl-6 protein was not significantly increased. The mRNA expression levels of Blimp-1 and Bcl-6 were significantly and positively correlated. Conclusion The expression of Blimp-1 and Bcl-6 in decidual tissue of patients with URSA increased.