药物制剂中N-乙酰半胱氨酸、青霉胺及巯丙酰甘氨酸的比色测定法

• 作 者: 程荣莉
• 出 处: 国外医药.合成药.生化药.制剂分册
• 发表日期: 1984年03期
• 关键词: 比色测定法 N-乙酰半胱氨酸 青霉胺 药物制剂 分光光度法测定 丙酰 校准曲线 巯基化合物 显色液 标准偏差

本文介绍药物制剂中 N-乙酰半胱氨酸(Ⅰ)、青霉胺(Ⅱ)及巯丙酰甘氨酸(Ⅲ)的比色测定法。本法基于巯基化合物在1,10-菲绕啉存在下,还原 Fe~(+++)成为 Fe~(++)的能力。形成的 Fe~(++)迅速定量地转化为稳定的三(1,10-菲绕啉)Fe~(++)络合物,在515nm 用分光光度法测定。各种商品制剂所得结果表明,本法具有快速、灵敏等特点。准确性(回收率99.5～101%)及精密度(相对标准偏差±0.6～1.6%)均良好。

This article describes the colorimetric assay of N-acetylcysteine ​​(I), penicillamine (II) and thio-propionylglycine (III) in pharmaceutical preparations. This method is based on the ability of the thiol compound to reduce Fe ~ (+++) into Fe ~ (++) in the presence of 1,10-phenanthroline. The formed Fe ~ (++) was rapidly and quantitatively converted into a stable tris (1,10-phenanthroline) Fe ~ (++) complex and was determined spectrophotometrically at 515 nm. The results obtained from various commercial preparations show that this method is fast and sensitive. Accuracy (99.5 ~ 101% recovery) and precision (relative standard deviation ± 0.6 ~ 1.6%) were good.