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目的:探讨魔芋提取物(KE)对高脂饮食大鼠胰岛素敏感性的影响.方法:利用高脂饮食(HFD)胰岛素抵抗(IR)大鼠模型,观察KE对IR的治疗作用,二甲双胍为对照药物.HFD饲养正常Wistar大鼠4周,分别以KE和二甲双胍灌胃治疗4周,观察动物的摄食量,饮水量和大便变化情况,并用葡萄糖氧化酶法测定空腹血糖,双抗体放免法测定血浆胰岛素,生化方法测定血脂(TC,TG,和HDL-C),并计算LDL-C,采用改良蒽酮法测定组织糖原,甘油磷酸氧化酶法测定组织中甘油三酯及改良的葡萄糖-胰岛素耐量试验测定胰岛素敏感性(K值).结果:HFD饲养4周时,与正常对照组比较,HFD组血糖,血清胰岛素,血脂明显升高,肝脏和肌肉中TG含量均明显升高(P<0.01),糖原含量显著下降(P<0.01),与正常对照组(K=8.3±0.7)比较,胰岛素敏感性下降(K=5.2±0.9,P<0.01).用KE(1.5,3.0 g·g~(-1)d~(-1))和二甲双胍(0.1 g·g~(-1)·d~(-1))治疗4周后,与HFD组比较治疗组胰岛素敏感性改善,K值分别为6.1±0.5,5.9±0.6和6.5±0.8(P<0.05),组织糖原含量明显增加(P<0.01),TC,TG,LDL-C显著降低(P<0。05),HDL-C升高不明显,HDL-C/TC值明显升高(P<0.05).结论:KE能在一定程度上阻止IR的发生和发展,具有提高HFD大鼠胰岛素敏感性的作用,其机制可能与其促进糖原合成和降低血脂有关.
Objective: To investigate the effect of konjac extract (KE) on insulin sensitivity of high-fat diet rats. Methods: To observe the therapeutic effect of KE on IR in a rat model of high-fat diet (HFD) insulin resistance (IR). Metformin was used as a control. Drugs.HFD Normal Wistar rats were fed for 4 weeks and were treated with KE and metformin for 4 weeks, respectively. Animals were observed for food intake, water intake, and stool changes. Glucose oxidase method was used to measure fasting blood glucose. Dual antibody antibody method was used to measure plasma. Insulin, blood lipids (TC, TG, and HDL-C) were measured by biochemical methods, LDL-C was calculated, tissue glycogen was determined by modified indolinone method, triglyceride in tissue and modified glucose-insulin were measured by glycerol phosphate oxidase method. Insulin sensitivity (K value) was determined by tolerance test. Results: Compared with the normal control group, the blood glucose, serum insulin, and serum lipids in the HFD group were significantly higher than those in the normal control group, and the TG content in the liver and muscle was significantly increased (P< 0.01), glycogen content was significantly decreased (P <0.01), compared with the normal control group (K = 8.3 ± 0.7), insulin sensitivity decreased (K = 5.2 ± 0.9, P <0.01). With KE (1.5, 3.0 g ·4 weeks after treatment of g~(-1)d~(-1)) and metformin (0.1 g·g~(-1)·d~(-1)), compared with HFD The insulin sensitivity of the group improved, K values were 6.1±0.5, 5.9±0.6, and 6.5±0.8 (P<0.05), and the tissue glycogen content was significantly increased (P<0.01), and TC, TG, and LDL-C were significantly decreased (P<0.05). <0. 05), HDL-C did not increase significantly, HDL-C/TC values increased significantly (P <0.05). Conclusion: KE can prevent the occurrence and development of IR to a certain extent, with the increase of insulin in HFD rats. The mechanism of sensitivity may be related to its promotion of glycogen synthesis and lowering of blood lipids.