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本文报道用HPLC法测定血浆或制剂中的氟尿嘧啶(5-FU),以胞啶为内标,该方法快速、精密.5-FU和内标的理论塔板数分别为242和208,其分离度为4.65,测定与5-FU联合化疗的其他抗癌药物均不干扰,用于制剂分析相对标准偏差<3.5%.方法:HPLC法,流动相为0.025mol/L正己烷磺酸水溶液,流速4ml/min,色谱柱内装10μm8mm×10cm MB~(TM) C_(??),检测波长为270nm.血浆中5-FU标准曲线:由于5-FU血浆中存在浓度范围宽,所以其标准曲线0.025～1.0μg/ml用于低浓度范围,1～25μg/ml用于高浓度范围.取空白血浆1ml,加适量0.1μg/ml或0.02μg/ml的5-FU水溶液,在上述浓度范围内使呈不同浓度.加色谱级纯
This article reports the determination of 5-fluorouracil (5-FU) in plasma or preparations by HPLC, with cytidine as internal standard, the method is rapid and precise.The theoretical plate numbers of 5-FU and internal standard are 242 and 208, respectively, Was 4.65, and the other anticancer drugs which were determined with 5-FU combined chemotherapy were not disturbed, and the relative standard deviation was less than 3.5% for the preparation analysis.METHODS: The HPLC method was as follows: the mobile phase was 0.025mol / L n-hexanesulfonic acid aqueous solution, the flow rate was 4ml / min, and the detection wavelength was set at 270 nm.The 5-FU standard curve in plasma: Because of the wide concentration range in 5-FU plasma, the standard curve of 0.025 ~ 1.0μg / ml for low concentration range, 1 ~ 25μg / ml for high concentration range, take blank plasma 1ml, add appropriate amount of 0.1μg / ml or 0.02μg / ml of 5-FU aqueous solution in the above concentration range to make Different concentrations. Plus pure chromatography grade