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采用化学阻断剂6-羟多巴胺(6-OHDA)损毁交感神经,研究了交感神经对小鼠小肠局部细胞免疫作用的内在机理。将30只雄性昆明小鼠随机分为2组,一组连续5d腹腔注射6-OHDA,另一组用作对照。采用免疫组织化学染色和细胞培养方法检测两组动物小肠CD4+、CD8+T淋巴细胞数量及肠系膜淋巴结T淋巴细胞体外增殖能力。结果表明,试验组的CD4+T细胞数量比对照组少6.5%～51.5%,十二指肠、空肠段差异极显著(P<0.01);CD8+T细胞数量比对照组增加5.6%～22.7%,十二指肠段差异显著(P<0.01);CD4+/CD8+T淋巴细胞的比值总体呈下降趋势。肠系膜淋巴结T淋巴细胞转化率降低,在ConA浓度为3μg/ml时比对照组降低了9.2%,差异显著(P<0.05)。以上结果提示:交感神经通过调节小鼠小肠T淋巴细胞增殖以及亚型CD4+、CD8+T淋巴细胞的数量,影响肠黏膜细胞免疫的调控。
The chemical blocker 6-hydroxydopamine (6-OHDA) destroyed the sympathetic nerve, and investigated the intrinsic mechanism of sympathetic nerve on local cellular immunity in mice. Thirty male Kunming mice were randomly divided into two groups. One group received intraperitoneal injection of 6-OHDA for 5 days, and the other group served as control. Immunohistochemical staining and cell culture method were used to detect the numbers of CD4 + and CD8 + T lymphocytes in small intestine and the proliferation of T lymphocytes in mesenteric lymph nodes in both groups. The results showed that the number of CD4 + T cells in the experimental group was 6.5% ~ 51.5% less than that in the control group (P <0.01). The number of CD8 + T cells increased 5.6% ~ 22.7 %, And duodenal segments (P <0.01). The ratio of CD4 + / CD8 + T lymphocytes decreased in general. The conversion of T lymphocytes in mesenteric lymph nodes decreased, with a significant difference of 9.2% (P <0.05) when the concentration of ConA was 3μg / ml. The above results suggest that sympathetic nerves can affect the regulation of intestinal mucosal cellular immunity by regulating the proliferation of T lymphocytes and the number of subtypes of CD4 + and CD8 + T lymphocytes in mice.