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A general functionalization strategy was reported,which enables one to conjugate saccharide(SA) on gold nanoparticle(GNP) surface without affecting SA properties.First,disulfide phenylboronic acid(Bor) functionalized GNPs(Bor@GNPs) were synthesized by the reaction of citrate stabilized GNPs of 13 nm in diameter with the mixture of Bor and pentapeptide(Cys-Ala-Leu-Asn-Asn,CALNN).Subsequently,the SA-GNP conjugates(SA@GNPs) were prepared by coupling SA to the GNP surface via the reaction of phenylboronic acid(PBA) with the cis-diol configuration in SA.The interactions of three SA@GNPs with three lectins have been analyzed by UV-visible spectroscopic and transmission electronic microscopic(TEM) techniques,respectively.The experimental results demonstrate that SA@GNPs can efficiently bind to lectins and show a great promise as optical probes for monitoring specific affinities of lectins for SA,and detecting lectins with high sensitivity.
A general functionalization strategy was reported, which enables one to conjugate saccharide (SA) on gold nanoparticle (GNP) surface without affecting SA properties. First, disulfide phenylboronic acid (Bor) functionalized GNPs stabilized GNPs of 13 nm in diameter with the mixture of Bor and pentapeptide (Cys-Ala-Leu-Asn-Asn, CALNN). Substituted, the SA- GNP conjugates (SA @ GNPs) were prepared by coupling SA to the GNP surface via the reaction of phenylboronic acid (PBA) with the cis-diol configuration in SA. interactions of three SA @ GNPs with three lectins have been analyzed by UV-visible spectroscopic and transmission electronic microscopic (TEM) techniques, respectively. that SA @ GNPs can efficiently bind to lectins and show a great promise as optical probes for monitoring specific affinities of lectins for SA, and detecting lectins with high sensitivity.