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有关恶性疟原虫体外培养配子体的报导,最早见于国外Smalley(1976年),在他的恶性疟原虫培养物中见到配子体,随后国外不少学者陆续报导培养配子体的成功。国内近年来也有学者对体外培养恶性疟原虫配子体进行研究,但至今尚无成功报导。我们于1989年开展此项工作,采用改变营养液成份和静止培养的方法,成功地培养出恶性疟原虫配子体。现将实验结果报告如下。1 材料与方法1.1 试剂与药品恶性疟原虫系FCC—1,由上海第二军医大学引进;RPMI1640粉为日本进口;Hepes为西德进口;次黄嘌呤为瑞士进口;红细胞采自健康人“O”型血,经玻珠脱纤并洗涤3次;血清为兔血清,由本所动物室饲养健康家兔经无菌采血分离
The first report of P. falciparum cultivation of gametophytes in vitro was first seen in Smalley abroad (1976). Gametophyte was seen in his P. falciparum culture. Subsequently, many scholars abroad reported the successful cultivation of gametophytes. In recent years, some scholars have also studied the in vitro cultivation of Plasmodium falciparum gametophytes, but so far no successful reports have been made. We carried out this work in 1989 and successfully cultivated Plasmodium falciparum gametocytes by changing the composition of nutrient solution and static culture. The experimental results are reported below. 1 Materials and Methods 1.1 Reagents and Drugs Plasmodium falciparum FCC-1, introduced by Shanghai Second Military Medical University; RPMI1640 powder imported from Japan; Hepes imported from West Germany; hypoxanthine imported from Switzerland; red blood cells collected from healthy people “O ”Type blood, defibrillation and washed three times by a glass bead; Serum rabbit serum, the animal room by the Institute of healthy rabbits separated by sterile blood separation