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目的观察运动疲劳对大鼠海马和纹状体脑源性神经营养因子(BDNF)及胶质纤维酸性蛋白(GFAP)表达的影响,探讨运动疲劳产生的神经生物学机制。方法:选取24只SD大鼠随机分为对照组(CG)和实验组(EG),分别在运动后0,12,24 h将大鼠麻醉,灌注制备脑石蜡切片,采用免疫组化染色法观察并分析海马和纹状体BDNF、GFAP表达水平的变化。结果EG海马锥体细胞排列紊乱疏松,部分细胞出现固缩、肿胀和碎裂;GFAP阳性细胞胞体增大,突起分支增多、增粗,着色加深。海马和纹状体12EG、24EG组BDNF阳性表达显著高于CG(P<0.05,P<0.01),且海马24EG显著高于0EG(P<0.05)。各EG大鼠海马区神经细胞GFAP阳性表达较CG明显增强(P<0.05,P<0.01)。结论运动疲劳引起大鼠海马和纹状体BDNF和GFAP表达水平上调,提示BDNF与GFAP参与了运动疲劳产生的神经生物学调控过程。
Objective To observe the effect of exercise-induced fatigue on the expression of brain-derived neurotrophic factor (BDNF) and glial fibrillary acidic protein (GFAP) in the hippocampus and striatum of rats and to explore the neurobiological mechanism of exercise-induced fatigue. Methods: Twenty-four SD rats were randomly divided into control group (CG) and experimental group (EG). Rats were anesthetized and anesthetized at 0, 12 and 24 hours after exercise. Cerebral paraffin sections were prepared by perfusion. Immunohistochemical staining The changes of BDNF and GFAP expression in hippocampus and striatum were observed and analyzed. RESULTS: The pyramidal cells in hippocampus of EG were loosely arranged, and some cells were pyknotic, swollen and fragmented. The number of GFAP positive cells increased, the number of protruding branches increased, the thickness increased and the coloration deepened. The positive expression of BDNF in hippocampus and striatum of 12EG and 24EG group was significantly higher than CG (P <0.05, P <0.01), and the 24EG of hippocampus was significantly higher than that of 0EG (P <0.05). Compared with CG, the positive expression of GFAP in hippocampus of EG rats significantly increased (P <0.05, P <0.01). Conclusion Exercise-induced fatigue induces up-regulation of BDNF and GFAP expression in hippocampus and striatum, suggesting that BDNF and GFAP are involved in the neurobiology regulation during exercise-induced fatigue.