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[目的]B.t.防治鳃金龟幼虫存在杀虫效果慢、幼虫死亡率低的缺点,试验目的是提高B.t.对鳃金龟幼虫的杀虫活性。[方法]应用荧光增白剂FB28处理华北大黑鳃金龟和暗黑鳃金龟围食膜,并通过FB28与B.t.对暗黑鳃金龟进行生物测定。[结果]荧光增白剂FB28能解离华北大黑鳃金龟和暗黑鳃金龟围食膜,分别至少有11条和8条蛋白多肽被解离,且随处理时间的增长,蛋白被解离的量增大,围食膜被破坏程度增大。FB28与B.t.一起使用,对其具有明显的增效作用。[结论]围食膜蛋白主要是几丁质结合蛋白,FB28可使几丁质结合蛋白从围食膜上解离下来,FB28对B.t.杀虫具有增效作用,为应用荧光增白剂作为增效因子防治鳃金龟幼虫提供了理论依据。
[Objective] The prevention and control of B. griseotacum larvae had the disadvantages of slow insecticidal effect and low larval mortality. The purpose of the experiment was to increase the insecticidal activity of B.t. on the gillot larvae. [Method] Fluorescent whitening agent FB28 was used to deal with the peritrophic membrane of Macrocephalus gossypii and Diabrillidae in North China, and the bioassay was carried out by FB28 and B.t. [Result] Fluorescent whitening agent FB28 could dissociate the peritrophic membrane of Scipex nigra and Scirpus mandshurica, at least 11 and 8 proteolytic peptides were dissociated, and the protein was dissociated with the increase of treatment time Increasing the amount of periocular membrane damage is increased. FB28 used together with B.t. has obvious synergies. [Conclusion] The peritrophic membrane protein is mainly chitin binding protein, FB28 can dissociate the chitin binding protein from the peritoneal membrane, and FB28 has a synergistic effect on Bt insecticide. For the application of optical brightener Efficacy of gill beetle larvae provide a theoretical basis.