论文部分内容阅读
目的:制备呕吐毒素(DON)的多克隆抗体,并对抗体效价、特异性和抑制率进行鉴定。方法:采用EDC法合成了呕吐毒素的完全抗原DON-BSA,并通过免疫新西兰大白兔,得到呕吐毒素的多克隆抗体。结果:ELISA检测出多抗中含有呕吐毒素的特异性抗体,其效价最高为12800,其抑制率为50%时,DON的浓度为10mg/L,DON的最低检测浓度为0.1mg/L。与结构类似物T-2毒素和NIV的交叉率分别为9.1%和4.9%。结论:呕吐毒素经过衍生化后制备出完全抗原,经免疫新西兰大白兔后得到的多克隆抗体可以完全满足ELISA检测的需要。
Objective: To prepare polyclonal antibodies against DON and to identify the antibody titer, specificity and inhibition rate. Methods: DON-BSA, the complete antigen of DON, was synthesized by EDC method. Polyclonal antibody of DON was obtained by immunizing New Zealand white rabbits. Results: The specific antibody against DON was detected by ELISA with the highest titer of 12800. When the inhibition rate was 50%, the concentration of DON was 10 mg / L and the minimum detectable concentration of DON was 0.1 mg / L. The crossover rates with structural analogue T-2 toxin and NIV were 9.1% and 4.9%, respectively. CONCLUSION: The deoxynivalenol is derivatized to produce the complete antigen. The polyclonal antibody obtained after immunization of New Zealand white rabbits completely meets the need of ELISA.