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采用丫啶橙（AO）荧光染色、流式细胞术和电镜观察非甾体类抗炎药消炎痛诱导人胃癌细胞株MKN28的凋亡。结果发现：经消炎痛作用2h后，MKN28细胞的AO染色阳性率上升，并和消炎痛呈剂量依赖关系，提示消炎病可诱导MKN28细胞凋亡，且凋亡的诱导在2h内即可启动。流式细胞未显示细胞周期亦发生变化，C0／G1期细胞从60．1％下降至28．6％，S期从28．5％增加至35．6％，G2/M期细胞从11．4％增加至35．8％。超微结构的变化为：细胞变圆，细胞核浓缩，染色质越边缘化分布。提示非衡作类抗炎药消炎病可诱导人胃癌细胞株MKN28凋亡，且凋亡的诱导在较短时间内即可完成，并对其机理进行探讨。
Acridine orange (AO) fluorescence staining, flow cytometry, and electron microscopy were used to observe the apoptosis of human gastric cancer cell line MKN28 induced by indomethacin, a non-steroidal anti-inflammatory drug. The results showed that: after 2 hours of indomethacin treatment, the positive rate of AO staining of MKN28 cells increased, and showed a dose-dependent relationship with indomethacin, suggesting that anti-inflammatory disease can induce apoptosis of MKN28 cells, and the induction of apoptosis can be initiated within 2 hours. Flow cytometry did not show changes in cell cycle, with C0/G1 phase cells decreasing from 60.1% to 28.6%, S phase from 28.5% to 35.6%, and G2/M phase cells from 11. 4% increased to 35.8%. The changes in ultrastructure are: rounding of cells, condensation of nuclei, and distribution of chromatin to the periphery. It is suggested that the anti-inflammatory drugs of unbalanced anti-inflammatory drugs can induce apoptosis of human gastric cancer cell line MKN28, and the induction of apoptosis can be completed in a short time, and its mechanism is discussed.