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探讨补牺对克山病病区居民血小板聚集和释放反应的影响。应用比浊法和ＥＬＩＳＡ对比测定病区补硒（２００μｇＳｅ／ｄ，亚硒酸钠，１２周）和未补硒居民血小板聚集率和卜血小板球蛋白（βＴＧ），用２，３－二氯氨萘荧光法和谷胱甘肽还原酶偶联法测定硒含量和ＧＳＨ－ＰＸ活性。结果表明：（１）补硒组红细胞硒含量和ＧＳＨ－ＰＸ活性显著高于对照组（Ｐ＜０．０１），（２）补硒组ＡＤＰ或凝血酶诱导的血小板聚集率显著低于对照组（Ｐ＜０．０５或０．０１）；（３）补硒组血浆βＴＧ水平以及血小板βＴＧ含量和释放率与对照组无显著差异（Ｐ＞０．０５）；（４）红细胞硒含量或ＧＳＨ－Ｐｘ活性与ＡＤＰ诱导的血小板聚集率呈显著负相关（ｒ＝０．３９０和－０．３７２，Ｐ＜０．０５），与凝血酶诱导的血小板聚集率呈不显著负相关（ｒ＝－０．２７９和－０．３０９，Ｐ＞０．０５）．提示补硒使病区低硒居民硒状态提高的同时，可降低其血小板聚集功能，但对血小板βＴＧ释放反应无显著影响。
To explore the influence of sacrifice on platelet aggregation and release in Keshan disease area residents. Nephelometry and ELISA were used to compare the platelet aggregation rate and the content of thromboplastin (βTG) in selenium (200μgSe / d, sodium selenite, 12 weeks) Determination of Selenium Content and GSH-PX Activity by Naphthalene Fluorescence and Glutathione Reductase Coupling Methods. The results showed that: (1) Selenium content and GSH-PX activity of erythrocytes in selenium-treated group were significantly higher than those in control group (P <0.01); (2) ADP or thrombin-induced platelet aggregation rate in selenium- (P <0.05 or 0.01). (3) There was no significant difference between the selenium group and the control group (P> 0.05). (3) Selenium content or GSH There was a significant negative correlation between -Px activity and ADP-induced platelet aggregation rate (r = 0.390 and -0.372, P <0.05), and no significant negative correlation with thrombin-induced platelet aggregation rate (r = 0.279 and -0.309, P> 0.05). It suggested that supplementing selenium could improve the selenium status of low-selenium residents and reduce its platelet aggregation function, but had no significant effect on platelet βTG release.