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目的探讨miR-183抑制结肠癌细胞SW620侵袭和迁移能力的作用是否需要NF-κB信号通路的参与性研究。方法采用脂质体转染细胞,高表达或沉默miR-183基因和Ezrin基因,用Western blot分析细胞核内p65蛋白表达的改变,用Transwell实验观察细胞侵袭和迁移能力的变化。结果在结肠癌SW620细胞中,高表达miR-183的靶基因Ezrin蛋白的表达,细胞核内NF-κB亚基p65蛋白的表达上调;抑制Ezrin蛋白的表达,能够抑制p65的表达。高表达miR-183能够抑制细胞核内p65蛋白的表达;抑制miR-183的表达,细胞核内p65的表达上调。p65蛋白的细胞核内定位特异性抑制剂PDTC能够抑制由于miR-183降低或Ezrin蛋白表达上调引起的细胞侵袭和迁移能力改变。结论 miR-183抑制结肠癌细胞SW620侵袭和迁移能力的作用需要NF-κB信号通路的参与。
Objective To investigate whether miR-183 suppresses the invasion and migration of human colon cancer cell line SW620 in a participatory manner. Methods Lipofectamine was used to transfect cells, miR-183 gene and Ezrin gene were highly expressed or silenced. The expression of p65 protein in the nucleus was analyzed by Western blot. Transwell assay was used to observe the changes of cell invasion and migration. Results The expression of Ezrin protein, a target gene of miR-183, and the expression of NF-κB subunit p65 in the nucleus of SW620 cells were up-regulated. The inhibition of Ezrin protein expression could inhibit the expression of p65 protein. High expression of miR-183 can inhibit the expression of p65 protein in the nucleus, inhibit the expression of miR-183, and up-regulate the expression of p65 in the nucleus. PDTC, a nuclear localization specific inhibitor of p65, is able to inhibit cell invasion and migration abilities due to the decrease of miR-183 or the upregulation of Ezrin protein. Conclusions The role of miR-183 in inhibiting invasion and migration of colon cancer cell line SW620 requires the involvement of NF-κB signaling pathway.