【摘 要】
：
The mouse model of oxygen induced retinopathy is suitable for the study of various retinal neovascularization diseases,including retinopathy of prematurity.The maternally expressed gene 3(MEG3)has been demonstrated to have an inhibitory effect on diabetic
【机 构】
：
Department of Ophthalmology,Shengjing Hospital of China Medical University,Shenyang,Liaoning Provinc
论文部分内容阅读
The mouse model of oxygen induced retinopathy is suitable for the study of various retinal neovascularization diseases,including retinopathy of prematurity.The maternally expressed gene 3(MEG3)has been demonstrated to have an inhibitory effect on diabetic retinopathy.In this study,we investigated the role of MEG3 overexpression in oxygen-induced retinopathy in mice.The results showed that MEG3 overexpression effectively inhibited the production of retinal neovascularization in oxygen-induced retinopathy mice.It acts by down-regulating the expression of phosphoinositide 3-kinase,serine/threonine kinase,and vascular endothelial growth factor and pro-inflammatory factors.MEG3 overexpression lentivirus has a future as a new method for the clinical treatment of retinopathy of prematurity.The animal experiments were approved by the Animal Ethics Committee of Shengjing Hospital of China Medical University,China(approval No.2016PS074K)on February 25,2016.
其他文献
At present, predicting the severity of brain injury caused by global cerebral ischemia/reperfusion injury (GCI/RI) is a clinical problem. After such an injury, clinical indicators that can directly reflect neurological dysfunction are lacking. The change
The mouse model of multiple cerebral infarctions, established by injecting fluorescent microspheres into the common carotid artery,is a recent development in animal models of cerebral ischemia. To investigate its effectiveness, mouse models of cerebral in
We recently achieved significant functional recovery after a complete spinal cord injury, allowing previously paralyzed mice to walk again. This was accomplished by a single, unilateral application of an adeno-associated virus (AAV) carrying the cDNA for
Due to the high degree of conservation of genes and mechanisms, the fruit fly Drosophila melanogaster is a powerful experimental in vivo tool to investigate complex diseases, thus complementing traditional vertebrate systems. Drosophila is also an advanta
Morphological analyses are key outcome assessments for nerve regeneration studies but are historically limited to tissue sections.Novel optical tissue clearing techniques enabling three-dimensional imaging of entire organs at a subcellular resolution have
Autophagy has been shown to play an important role in Parkinson\'s disease.We hypothesized that skin-derived precursor cells exhibit neuroprotective effects in Parkinson\'s disease through affecting autophagy.In this study,6-hydroxydopamine-damaged SH
The mechanism of AD remains uncovered:The current mainstream doctrine in Alzheimer\'s disease (AD) is the amyloid cascade hypothesis. According to this hypothesis, amyloid-β (Aβ) deposition is the main reason for neurofibrillary tangles formation and sy
Severe cerebral ischemia/reperfusion injury has been shown to induce high-level autophagy and neuronal death. Therefore, it is extremely important to search for a target that inhibits autophagy activation. Long non-coding RNA MEG3 participates in autophag
The nervous system is a highly complex organization composed of its basic unit, the neuronal cells, and specialized supporting cells, the glial cells. The main glial cells in the central nervous system (CNS) are astrocytes, microglia and oligodendrocytes.
Mutations that cause protein misfolding are implicated in conditions such as retinitis pigmentosa (RP), Usher Syndrome, and myocilin associated primary open angle glaucoma. The aggregation and continuous degradation of a highly abundant misfolded protein