论文部分内容阅读
目的:观察不同免疫缺陷小鼠中的人肝癌细胞生长情况以及T,B淋巴细胞的免疫作用,探讨免疫缺陷小鼠肿瘤模型制作的意义。 方法:将体外培养的人肝癌细胞接种到四种免疫缺陷小鼠,分别为:B细胞缺陷的CBA/N,T细胞缺陷的Balb/c-nu,T,B细胞缺陷的SCID及免疫重建的SCID小鼠,观察其生长特点;作鼠脾细胞毒试验,测定外周血CD4~+,CD8~+数分数和荷瘤鼠血清Ig含量的变化;作肝癌细胞凝集试验。 结果:CBA/N和用BALB/c鼠外周血淋巴细胞重建的SCID(B-PBL-SCID)鼠不成瘤,nude、SCID和用CBA/N鼠外周血淋巴细重建的SCID(C-PBL-SCID)小鼠全部成瘤;SCID鼠的瘤体比裸鼠瘤体长的更快,肝内接种转移率更高、转移范围更大。接种瘤细胞的BALB/c和CBA/N鼠脾细胞对癌细胞杀伤力较强,免疫重建的SCID鼠脾细胞毒杀伤较小。接种瘤细胞的鼠CD4~+数分数都下降,CD8~+变化不大,CD4~+/CD8~+比值下降。具有B细胞的实验鼠均测得Ig在mg·L~(-1)水平,并能使癌细胞产生凝集反应。 结论:SCID鼠是建立肿瘤模型和免疫重建研究的理想小鼠;小鼠成瘤率与T细胞相关,T细胞在异种瘤移植排斥中起主要作用;B淋巴细胞及其产生的抗体在抗肿瘤中起着不可忽视的作用。
Objective: To observe the growth of human hepatoma cells in different immunodeficient mice and the immune function of T and B lymphocytes, and to explore the significance of making tumor models in immunodeficient mice. Methods: Human hepatoma cells cultured in vitro were inoculated into four kinds of immunodeficient mice, which were: B cell deficient CBA / N, T cell deficient Balb / c-nu, T and B cell deficient SCID and immune reconstructed SCID mice to observe their growth characteristics; for the murine splenic cytotoxicity test, determination of peripheral blood CD4 ~ +, CD8 ~ + number fraction and serum tumor Ig content; for liver cancer cell agglutination test. RESULTS: CBA / N and SCID (B-PBL-SCID) mice reconstituted with BALB / c mouse peripheral blood lymphocytes were not tumorigenic, nude, SCID and SCID reconstructed with CBA / N murine peripheral blood lymphocytes (C-PBL- SCID mice all formed tumors; the tumors of SCID mice grew faster than those of nude mice, with higher intrahepatic inoculation rates and larger metastatic potential. Inoculated tumor cells of BALB / c and CBA / N mouse spleen cells on the cancer cell cytotoxicity, immunohistochemical SCID murine spleen cytotoxicity smaller. Inoculated tumor cells of mice CD4 ~ + fractional decline, CD8 ~ + little change, CD4 ~ + / CD8 ~ + ratio decreased. All the mice with B cells measured Ig at mg · L -1 level and could agglutinate cancer cells. CONCLUSIONS: SCID mice are the ideal mice for the establishment of tumor models and immune reconstitution studies; the rate of tumorigenesis in mice is related to T cells; T cells play a major role in the rejection of xenograft tumors; B lymphocytes and the antibodies they produce play important roles in antitumor Plays a role that can not be ignored.