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对 4 3个基因型的未熟幼胚进行离体培养 ,研究不同基因型对离体培养的反应 ,不同质量浓度 2 ,4 D对胚性愈伤组织百分率的影响以及不同基因型愈伤组织的再生苗率 .结果表明 ,自交系A188,87 1、郑 2 2、综 3、综 31和掖 4 78以及它们的杂交种产生了胚性愈伤组织 ;继代过程中 ,2 ,4 D质量浓度降至 1mg·L-1提高了胚性愈伤组织百分率 ;4 3个基因型中仅部分基因型再生了植株 ,基因型是能否再生植株的主要因素 ,培养基成分也影响再生苗率 ,改良MS成分和低浓度的激动素有利于植株再生
The immature embryos of 43 genotypes were cultured in vitro to study the effects of different genotypes on the in vitro culture, the effects of different concentrations of 2,4 D on the percentage of embryogenic callus, and the effects of different genotype callus The results showed that embryogenic callus was generated in inbred lines A188, 87 1, Zheng 2 2, Zao 3, Zu 31, and Ye 4 78 and their hybrids. In the process of subculture, 2,4 D Reducing the concentration to 1 mg · L -1 increased the percentage of embryogenic callus; only some of the 4 genotypes regenerated the plants, and the genotypes were the main factors that could lead to plant regeneration, and the composition of the medium also affected the regeneration of seedlings Rates, improved MS composition, and low concentrations of kinetin favor plant regeneration