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目的探讨选择性环氧合酶-2(Cyclooxygenase-2,COX-2)抑制剂塞来昔布(Celecoxib)对白介素-1β(Interleukin-1β,IL-1β)诱导的肺癌A549细胞前列腺素E2(PGE2)和血管内皮生长因子(vascularendothelialgrowthfactor,VEGF)蛋白表达的影响。方法2005年1月至5月在中南大学湘雅二医院呼吸科,采用不同浓度的塞来昔布干预IL-1β诱导的A549细胞,逆转录聚合酶链反应(reversetranscription-polymerasechainrection,RT-PCR)半定量方法测定A549细胞VEGFmRNA的相对量,酶联免疫吸附试验(enzyme-linkedimmunosorbentassay,ELISA)法测定培养上清液中PGE2(ProstaglandinE2)及VEGF蛋白浓度。结果不同浓度的塞来昔布干预后,IL-1β诱导的A549细胞VEGFmRNA和蛋白质表达、PGE2分泌较非干预组浓度降低,差异有显著性(P<0.05);随着塞来昔布浓度的增高,VEGF蛋白表达、PGE2分泌量亦逐渐下降,各组间差异亦有显著性(P<0.05);PGE2和VEGF蛋白质下降的变化趋势相似。结论塞来昔布呈剂量依赖性下调IL-1β诱导的A549细胞VEGFmRNA和蛋白质的表达,其机制可能是通过抑制PGE2合成而实现的。
Objective To investigate the effect of Celecoxib, a selective COX-2 inhibitor, on the expression of prostaglandin E2 in human lung adenocarcinoma A549 cells induced by interleukin-1β (IL-1β) (PGE2) and vascular endothelial growth factor (VEGF) protein expression. Methods From January 2005 to May 2005, IL-1β-induced A549 cells were treated with different concentrations of celecoxib in the respiratory department of the Second Xiangya Hospital of Central South University. Reverse transcriptase polymerase chain reaction (RT-PCR) Semi-quantitative method was used to determine the relative amount of VEGF mRNA in A549 cells. Prostaglandin E2 and VEGF protein concentrations in culture supernatants were determined by enzyme-linked immunosorbent assay (ELISA). Results After intervention with different concentrations of celecoxib, the expression of VEGF mRNA and protein in A549 cells induced by IL-1β was lower than that in the non-intervention group (P <0.05). With the increase of celecoxib concentration (P <0.05). The decreasing trend of PGE2 and VEGF proteins was similar. Conclusion Celecoxib can down-regulate the expression of VEGF mRNA and protein in A549 cells induced by IL-1β in a dose-dependent manner. The mechanism may be through the inhibition of PGE2 synthesis.