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目的:观察补肾活血方对家兔膝骨关节炎(OA)滑膜组织基因表达谱的调控作用。方法:Hulth方法建立家兔膝骨关节炎模型,给予补肾活血方干预、常规抽提和纯化RNA,采用AffymetrixRat230 2.0Array芯片检测滑膜组织的基因表达变化。结果:芯片结果显示补肾活血方对模型组滑膜组织基因表达具有明显调控作用,补肾活血方有明显上调作用的基因中,中药组比模型组上调1.8倍以上的有10个;具有明显下调作用的基因中,中药组比模型组下调2倍以上的有4个。结论:补肾活血方可能通过调控目前在骨关节炎发生、发展中作用尚不明确的基因,起到抑制滑膜病变进展、促进软骨修复,从而达到治疗骨关节炎作用。
Objective: To observe the regulation effect of Bushen Huoxue Recipe on the gene expression profile of knee osteoarthritis (OA) synovial tissue in rabbits. Methods: Rabbit knee osteoarthritis model was established by Hulth method. Bushenhuoxue prescription was given to the rabbits to routinely extract and purify RNA. The gene expression of synovial tissue was detected by Affymetrix Rat230 2.0Array chip. Results: The results of the chip showed that Bushen Huoxue Decoction significantly regulates the gene expression of synovial tissue in the model group, and among the genes of Bushen Huoxue Decoction significantly up-regulated in the model group, the TCM group has up-regulated more than 1.8-fold and 10-fold compared with the model group, Of the genes, the traditional Chinese medicine group than the model group more than twice as many as 4. Conclusion: Bushen Huoxue prescription may play an important role in the treatment of osteoarthritis by regulating the genes which are not clear in the occurrence and development of osteoarthritis, inhibiting the progression of synovial membrane disease and promoting the cartilage repair.