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选用了九种吸附剂,加或不加硫酸钙粘合剂,分别制成吸附薄层,先后筛选了139种展开剂系统,希望分离洋地黄叶中12种主要强心甙,但结果只能部分分离,效果不够理想。最后采用了分配薄层层离法,在硅藻土薄层上,以甲酰胺作固定相,成功地分离了各种强心甙,所得色点清晰集中,R_f值距离大.较好的展开剂系统有氯仿-丙酮-醋酸乙酯-甲酰胺(5:2:3:饱和);氯仿-丙酮-醋酸乙酯-乙醇-甲酰胺(8:2:0.5:0.5:饱和);氯仿-笨-醋酸乙酯-甲酰胺(1:2:3:饱和);氯仿-丁醇-甲酰胺(9:1:饱和)等.所用显色剂为50％硫酸,显色后各种强心甙及甙元呈现不同颜色,有助于鉴别.
Nine kinds of adsorbents were used, with or without calcium sulfate binder, made into thin layers of adsorption, and 139 kinds of developing systems were successively screened. It was hoped to separate 12 main cardiotonic plaques in digital yellow leaves, but the result was only Partial separation, the effect is not ideal. Finally, the distribution thin layer separation method was adopted. On the diatomite thin layer, formamide was used as the stationary phase, and various cardiac cesiums were successfully separated. The resulting color points were clearly concentrated, and the R_f value was widely spaced. The agent system includes chloroform-acetone-ethyl acetate-formamide (5:2:3:saturated); chloroform-acetone-ethyl acetate-ethanol-formamide (8:2:0.5:0.5:saturation); chloroform-styl Ethyl acetate-carboxamide (1:2:3:saturated); Chloroform-butanol-carboxamide (9:1:saturated). The chromogenic reagent used is 50% sulfuric acid. And Shan Yuan presents different colors to help identify.