【摘 要】
：
In an in vitro transcription system, the intact virion of rice ragged stunt virus (RRSV) coulddirect the synthesis of an acid-insoluble material. This indicate
【机 构】
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Shanghai Institute of Biochemistry, Academia Sinicarrrrrrrrn,Shanghai Institute of Biochemi
【出 处】
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Science in China,Ser.B
论文部分内容阅读
In an in vitro transcription system, the intact virion of rice ragged stunt virus (RRSV) coulddirect the synthesis of an acid-insoluble material. This indicated that the virus might contain RNA poly-merase activity, with an optimum range of activity at 30-40℃, maximal at 35℃. Divalent cationswere essential for the activity. Transcription products accumulated in the incubation period from 0 to4 h In addition, the polymerase activity declined if the virus was heat-shocked or treated with chy-motrypsin, which implied that no prior treatment was required to activate transcription catalyzed byRRSV.
In an in vitro transcription system, the intact virion of rice ragged stunt virus (RRSV) coulddirect the synthesis of an acid-insoluble material. This indicates that the virus might contain RNA poly-merase activity, with an optimum range of activity at 30- 40 ° C, maximal at 35 ° C. Divalent cationswere essential for the activity. Transcription products accumulated in the incubation period from 0 to 4 h In addition, the polymerase activity declined if the virus was heat-shocked or treated with chy-motrypsin, which implied that no prior treatment was required to activate transcription catalyzed byRRSV.
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