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Aim:To observe the preventive and therapeutic effects of Ginkgo biloba extract(GbE) on early experimental diabetic nephropathy (DN) in rats.Methods:After anearly DN model was induced by streptozotocin,rats were administered GbE at 3doses for 12 weeks.Fasting blood glucose,creatinine (Cr),blood urea nitrogen(BUN),urine protein,kidney index,anti-oxidase,advanced glycosylation end prod-ucts (AGE),collagen IV and laminin,matrix metalloproteinases-2 (MMP-2) and thetissue inhibitor of metalloproteinase-2 (TIMP-2),connective tissue growth factor(CTGF),and transforming growth factor-β1 (TGF-β1) mRNA were measured bydifferent methods.The ultrastructural morphology and the thickness of glomeru-lar base membrane (GBM) were observed by a transmission electron microscope.Results:For the GbE-treated DN rats,when compared with the vehicle-treatedDN rats,the fasting blood glucose level,Cr,BUN,urine protein level,and theintensity of oxidative stress were significantly decreased.The expression of MMP-2 greatly increased,and TIMP-2 decreased.Also,AGE,either in serum or in renal,the collagen IV,laminin,CTGF levels,and TGF-β1 mRNA were reduced.Furthermore,both relative grades of mesangium hyperplasia by microscopicalobservation and the thickness of GBM by electron microscope measurement de-creased significantly.Conclusion:GbE has protective effects on several pharma-cological targets in the progress of DN and is a potential drug for the preventionof early DN.
Aim: To observe the preventive and therapeutic effects of Ginkgo biloba extract(GbE) on early experimental diabetic nephropathy (DN) in rats.Methods:After anearly DN model was induced by streptozotocin,rats were administered GbE at 3doses for 12 weeks.Fasting blood Glucose,creatinine (Cr),blood urea nitrogen(BUN),urine protein,kidney index,anti-oxidase,advanced glycosylation end prod-ucts (AGE),collagen IV and laminin,matrix metalloproteinases-2 (MMP-2) and thetissue The inhibitor of metalloproteinase-2 (TIMP-2), connective tissue growth factor (CTGF), and transforming growth factor-β1 (TGF-β1) mRNA were measured by differentiated methods. The ultrastructural morphology and the thickness of glomeru-lar base membrane (GBM ) were observed by a transmission electron microscope.Results:For the GbE-treated DN rats,when compared with the vehicle-treatedDN rats,the fasting blood glucose level,Cr,BUN,urine protein level,and the intensity of oxidative stress were significantly decreased .The expression of MMP- 2 significantly increased, and TIMP-2 decreased.Also,AGE,either in serum or in renal,the collagen IV,laminin,CTGF levels,and TGF-β1 mRNA were reduced.Furthermore,both relative grades of mesangium hyperplasia by microscopical observation and the Thickness of GBM by electron microscope measurement de-creased significant.Conclusion:GbE has protective effects on several pharma-cological targets in the progress of DN and is a potential drug for the prevention of early DN.