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【目的】 检测真核生物蛋白合成启始因子4E(eIF-4E)在喉癌和下咽癌?癌旁组织?安全边缘和正常黏膜的表达,探讨在喉癌?下咽癌发生?发展中的意义?【方法】25例标本中,喉癌18例,下咽癌7例?采用RT-PCR和Western blot分析喉癌和下咽癌的肿瘤核心,癌旁组织,安全切缘及喉腔正常黏膜中eIF-4E的表达水平?【结果】 eIF-4E的mRNA和蛋白在正常黏膜(0.27±0.11和12.20±5.56)?安全边缘组织(0.27±0.10 和12.90±5.08)?癌旁组织(0.49±0.15和 36.32±10.42)和癌肿核心组织(0.82±0.25和75.81±20.77)的表达呈递增趋势?【结论】 eIF-4E在喉癌和下咽癌中均过度表达,可考虑作为喉癌和下咽癌分子水平的肿瘤标志?
【Objective】 To detect the expression of eukaryotic protein synthesis initiation factor 4E (eIF-4E) in laryngeal and hypopharyngeal carcinomas adjacent to the margins of normal tissues and normal mucosa, and to explore the development of laryngeal carcinoma and hypopharyngeal carcinoma. Significance [Method] Among 25 specimens, 18 cases of laryngeal carcinoma and 7 cases of hypopharyngeal carcinoma were analyzed for tumor core, paracancerous tissue, safety margin, and laryngeal cavity by RT-PCR and Western blot. The expression level of eIF-4E in normal mucosa?[Results] The mRNA and protein of eIF-4E were in the normal mucosa (0.27±0.11 and 12.20±5.56), the marginal tissues of security (0.27±0.10 and 12.90±5.08), and the adjacent tissues ( The expression of 0.49±0.15 and 36.32±10.42) and cancer core tissue (0.82±0.25 and 75.81±20.77) showed increasing trend. 【Conclusion】 eIF-4E is over-expressed in laryngeal cancer and hypopharyngeal cancer and may be considered as a throat. Cancer and hypopharyngeal cancer at the molecular level of tumor markers?