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目的：观察细胞因子对心脏微血管内皮细胞表面细胞间粘附分子－１（ｉｎｔｅｒｃｅｌｕｌａｒａｄｈｅｓｉｏｎｍｏｌｅｃｕｌｅ－１，ＩＣＡＭ－１）的表达的调控，及对内皮细胞与激活淋巴细胞粘附的影响。方法：体外培养大鼠心脏微血管内皮细胞，以肿瘤坏死因子（ｔｕｍｏｒｎｅｃｒｏｓｉｓｆａｃｔｏｒ－α，ＴＮＦ－α）、白细胞介素－６（ｉｎｔｅｒｌｅｕｋｉｎ６，ＩＬ－６）和白细胞介素－１（ｉｎｔｅｒｌｅｕｋｉｎ１β，ＩＬ－１β）诱导。采用免疫组织化学染色法观察内皮细胞表面ＩＣＡＭ－１表达；采用粘附试验和抗ＩＣＡＭ－１或抗ＬＦＡ－１单克隆抗体阻断抑制试验。结果：ＴＮＦ－α、ＩＬ－６和ＩＬ－１β诱导内皮细胞１８～２４ｈ，均可使内皮细胞与淋巴细胞的粘附率显著增加，ＴＮＦ－α和ＩＬ－１β的诱导还可使内皮细胞表面ＩＣＡＭ－１分子表达明显增强，表现为ＩＣＡＭ－１表达阳性细胞数增多，着色加深。用１０～２０ｍｇ／Ｌ抗ＩＣＡＭ－１或抗ＬＦＡ－１单克隆抗体均可部分抑制内皮细胞与淋巴细胞的粘附。结论：ＴＮＦ－α和ＩＬ－１β可以有效地激活心脏微血管内皮细胞，通过诱导内皮细胞ＩＣＡＭ－１表达增多，促进内皮细胞与淋巴细胞的粘附
OBJECTIVE: To investigate the effects of cytokines on the expression of intercellular adhesion molecule-1 (ICAM-1) on cardiac microvascular endothelial cells and on the adhesion of endothelial cells to activated lymphocytes. Methods: Rat cardiac microvascular endothelial cells were cultured in vitro. The effects of tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6) and interleukin 1β (IL- Induced. Immunohistochemical staining was used to observe the expression of ICAM-1 on the surface of endothelial cells. Adhesion and anti-ICAM-1 or anti-LFA-1 monoclonal antibodies were used to block the inhibition of ICAM-1 expression. Results: The adhesion rate of endothelial cells and lymphocytes was significantly increased after TNF-α, IL-6 and IL-1β induced endothelial cells for 18-24 h. The induction of TNF-α and IL-1β also induced the adhesion of endothelial cells to the surface of endothelial cells The expression of ICAM-1 was significantly increased, which showed that the number of ICAM-1 positive cells increased and the coloration deepened. With 10 ~ 20mg / L anti-ICAM-1 or anti-LFA-1 monoclonal antibodies can be partially inhibited endothelial cell adhesion to lymphocytes. Conclusion: TNF-α and IL-1β can effectively activate cardiac microvascular endothelial cells and promote the adhesion of endothelial cells to lymphocytes by inducing the expression of ICAM-1 in endothelial cells