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目的 :探讨狼疮性肾炎 (LN)患者谷胱甘肽转移酶 μ(GSTμ)基因缺失与氧化损伤的关系。 方法 :应用化学分析法检测 5 1例LN患者和 40例健康对照者血中一氧化氮 (NO)、脂质过氧化物 (LPO)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶 (GSH px)和谷胱甘肽 (GSH) ,用PCR法检测GSTμ基因。 结果 :LN组NO、LPO明显增高 ,SOD、GSH px、GSH明显降低。NO与LPO呈显著正相关 ,与SOD、GSH px、GSH呈显著负相关。NO、LPO与补体 3 (C3)呈显著负相关 ,与抗ds DNA、血清γ球蛋白呈显著正相关。SOD、GSH px、GSH与C3呈显著正相关 ,与抗ds DNA、血清γ球蛋白呈显著负相关。LN组GSTμ基因缺失率达 70 .5 9% ,明显高于对照组 (47.5 0 % )。GSTμ基因缺失的LN患者与GSTμ基因携带的LN患者比较 ,LPO明显增高 ,SOD、GSH px、GSH明显降低。结论 :LN患者存在氧化损伤和抗氧化能力降低 ,GSTμ基因缺失的LN患者更严重。GSTμ基因缺失可能是LN的易患因素之一。提示LN患者应给予抗氧化治疗
Objective: To investigate the relationship between glutathione transferase μ (GSTμ) gene deletion and oxidative damage in patients with lupus nephritis (LN). Methods: The levels of blood nitric oxide (NO), lipid peroxides (LPO), superoxide dismutase (SOD) and glutathione in 51 LN patients and 40 healthy controls were detected by chemical analysis. Oxidase (GSH px) and glutathione (GSH) were detected by PCR method GSTμ gene. Results: NO, LPO in LN group were significantly increased, while SOD, GSH px, GSH were significantly decreased. NO was positively correlated with LPO and negatively correlated with SOD, GSH px and GSH. NO, LPO and complement 3 (C3) showed a significant negative correlation with anti-ds DNA, serum gamma globulin was significantly correlated. There was a significant positive correlation between SOD, GSH px, GSH and C3, but negatively correlated with anti-ds DNA and serum gamma globulin. The deletion rate of GSTμ gene in LN group was 70.59%, which was significantly higher than that in control group (47.5%). LN patients with GSTμ gene deletion had significantly higher LPO and lower SOD, GSH px and GSH than LN patients with GSTμ gene. CONCLUSIONS: LN patients have decreased oxidative damage and antioxidant capacity, and patients with LN deficient in GSTμ gene are more severe. GSTμ gene deletion may be one of the predisposing factors for LN. Tip LN patients should be given anti-oxidative therapy