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贵农22是由簇毛麦、硬粒小麦以及普通小麦杂交选育而成的普通小麦品种,其对我国目前所有已知条锈菌生理小种均表现高度抗病。为了明确其抗条锈性遗传基础,并对抗条锈基因进行分子作图,本研究选用条锈菌重要小种CYR29、CYR30、CYR32、CYR33和Su11-11,对贵农22与条锈病感病品种辉县红或铭贤169杂交F2代、BC1F1代、BC1F2代进行抗锈性遗传分析,并对贵农22控制Su11-11抗病性的1对隐性基因进行SSR标记。结果表明,贵农22至少含有3对抗条锈病基因。利用272株贵农22/铭贤169 BC1 F2群体筛选到2个与贵农22控制Su11-11抗性的隐性基因连锁的SSR标记Xwmc44和Xcfa2147,遗传距离分别为5.1和7.3 cM,并将抗病基因定位于小麦1BL上,暂命名为YrGn22。基因来源、抗病性分析以及分子检测结果表明,YrGn22不同于1BL上的已知小麦抗条锈病基因Yr3、Yr9、Yr21和Yr29,可能是1个新基因。
Guinong 22 is a common wheat variety which is bred from the hybrids of Tamarix, Triticum and Triticum aestivum. It is highly resistant to all of the current races of P. striiformis in our country. In order to clarify the genetic basis of stripe rust resistance and molecular mapping against stripe rust genes, we selected CYR29, CYR30, CYR32, CYR33, and Su11-11, The F2 generation, BC1F1 generation and BC1F2 generation of F1 hybrid Huixianghong or Mingxian169 were used for genetic analysis of rust resistance, and 1 recessive gene of Guinong 22 controlling Su11-11 resistance was SSR-labeled. The results showed that Guinin 22 contains at least 3 stripe rust resistance genes. Two SSR markers, Xwmc44 and Xcfa2147, linked to the recessive gene controlling Su11-11 resistance of Guinong 22 were screened from 272 BC1 F2 populations from Guinong 22 / Mingxian 169 with genetic distances of 5.1 and 7.3 cM, respectively. The disease-resistance gene is located on wheat 1BL and tentatively named YrGn22. The origin of the genes, the disease resistance analysis and the molecular test results showed that YrGn22 might be a new gene different from the known wheat stripe rust resistance genes Yr3, Yr9, Yr21 and Yr29 on 1BL.