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护颖是水稻等稻族禾本科植物的小穗器官,关于其进化及发育的分子机制还不十分清楚。本研究报道了一个来源于保持系水稻‘西农1B’的EMS诱变群体中的护颖异常突变体,暂被命名为lsl(lemma-like sterile lemma),主要表现为护颖显著伸长变宽。花器官发育早期,lsl突变体护颖原基表现出与野生型护颖一致的发育分化模式;到成熟期,lsl突变体护颖表层细胞严重硅化,着生大量毛刺,与外稃类似,护颖内部细胞结构解剖也和外稃一致。这些结果表明突变体的护颖发生了向外稃的同源异型转化。将lsl与‘缙恢10号’杂交构建F1和F2定位群体。遗传分析表明该突变体受隐性单基因控制。利用SSR分子标记,通过BSA法,将LSL基因定位在第7染色体上SSR标记RM1085和RM6663之间,物理距离为735.5 kb。本研究为进一步理解LSL基因的功能及分子机理奠定了良好的基础。
Miriam is the spikelet organ of rice and other gramineous plants, and the molecular mechanisms of its evolution and development are not well understood. In this study, we report an abnormal mutants of EMS mutation derived from maintainer line “Xinnong 1B” in rice and temporarily named lsl (lemma-like sterile lemma) width. At the early stage of floral organ development, the lsl mutants showed the same pattern of developmental differentiation as the wild type. By the maturity stage, the lsl mutants were highly silicified and had a large number of glitches, similar to the lemma Internal anatomy of the cells is also consistent with the nephelium. These results suggest that outgrowth of homeopathic transformation of the mutant. F1 and F2 loci were constructed by crossing lsl with ’Jinhui 10’. Genetic analysis showed that the mutant was controlled by a recessive single gene. Using SSR molecular marker, the LSL gene was located on chromosome 7 by SSR markers RM1085 and RM6663 with a physical distance of 735.5 kb by BSA method. This study laid a good foundation for further understanding the function and molecular mechanism of LSL gene.