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目的：探讨弓形虫感染的淋巴结组织中的病原体检测。方法：用聚合酶链反应（ＰＣＲ）和基因工程技术制备弓形虫（ＲＨ株）特异ＤＮＡ克隆片段，以地高辛随机引物法标记克隆的弓形虫ＤＮＡ片段作为探针，与淋巴结组织切片中核酸原位杂交试验（ＩＳＨ）检测病理标本中的弓形虫ＤＮＡ。结果：３２例何杰金淋巴瘤（ＨＤ）和４１例非何杰金淋巴瘤（ＮＨＬ）病理标本中各有一例阳性，４７例慢性淋巴结炎（ＣＬ）病理标本中呈ＩＳＨ阳性者２例，总阳性率为３．３％（４／１２０）。研究证明，所制备的探针能检测１０ｐｇ的ＤＮＡ，且特异性强。结论：核酸原位杂交为弓形虫病的病理标本中病原体检测提供了可行的方法。
Objective: To explore the pathogen detection of Toxoplasma gondii infected lymph node tissue. Methods: DNA fragment of Toxoplasma gondii (RH strain) was prepared by polymerase chain reaction (PCR) and genetic engineering techniques. The DNA fragment of cloned Toxoplasma gondii was labeled with digoxigenin random primer, Toxoplasma gondii DNA was detected by in situ hybridization (ISH). Results: One case was positive in each of 32 cases of Hodgkin’s lymphoma (NHL) and 41 cases of non-Hodgkin’s lymphoma (NHL). Two cases of ISH were found in 47 cases of chronic lymphadenitis (CL) The total positive rate was 3.3% (4/120). Studies have shown that the prepared probe can detect 10pg of DNA, and the specificity is strong. Conclusion: In situ hybridization provides a feasible method for the pathogen detection of toxoplasmosis.