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目的:探讨miR-617在宫颈癌组织中的表达及其过表达对宫颈癌SiHa细胞增殖、凋亡、迁移等细胞生物学行为的影响。方法:采用实时定量PCR法检测宫颈鳞癌组织及正常宫颈组织中miR-617表达。宫颈癌SiHa细胞中转染miR-617模拟物使其过表达,采用CCK-8细胞增殖检测试剂盒检测细胞增殖活力,流式细胞术检测细胞凋亡,平板克隆实验检测细胞克隆形成率,划痕实验检测细胞迁移能力,Western blot法检测靶基因C-末端结合蛋白1(Ct BP1)蛋白表达水平。结果:miR-617在宫颈鳞癌组织中表达下调,过表达miR-617可抑制宫颈癌SiHa细胞增殖,促进SiHa细胞凋亡,抑制SiHa细胞的克隆形成能力,抑制细胞的迁移,过表达miR-617可降低CtBP1蛋白表达。结论:miR-617在宫颈鳞癌中表达下调,可抑制SiHa细胞增殖、促进细胞凋亡、抑制克隆形成和迁移,其作用机制可能与CtBP1蛋白下调有关。
Objective: To investigate the expression of miR-617 in cervical cancer and its effect on cell biology behavior such as proliferation, apoptosis and migration of cervical cancer SiHa cells. Methods: Real-time quantitative PCR was used to detect the expression of miR-617 in cervical squamous cell carcinoma and normal cervical tissues. The expression of miR-617 mimics was overexpressed in cervical cancer SiHa cells. The proliferation of SiHa cells was detected by CCK-8 cell proliferation kit. Cell apoptosis was detected by flow cytometry. The cell migration ability was detected by the scoring assay. The protein expression of Ct BP1 was detected by Western blot. Results: The expression of miR-617 was down-regulated in cervical squamous cell carcinoma. Overexpression of miR-617 inhibited the proliferation of SiHa cells, promoted the apoptosis of SiHa cells, inhibited the clonality of SiHa cells, inhibited the cell migration, over-expressed miR- 617 reduces CtBP1 protein expression. Conclusion: The down-regulation of miR-617 in squamous cell carcinoma of the cervix can inhibit the proliferation of SiHa cells, promote the apoptosis and inhibit the formation and migration of SiHa cells. Its mechanism may be related to the down-regulation of CtBP1 protein.