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自1957年 Simon Holdowsky 提出用纤维薄膜过滤法作抗菌素无菌检查以来,经不断改进,该方法已逐步趋于完善[药检工作通讯7(3):173,1966],而被中国药典1977年版一部所收载。薄膜法具有受检范围广、细菌检出灵敏度高、去活率高等优点。但本法的缺点是无菌操作要求高,否则容易染菌,造成实验失败。现提出操作中的注意点,供大家参考。1.薄膜在蔡氏漏斗(以下简称漏斗)中夹好后,用水湿润(避免高压后薄膜发脆,造成抽滤后取出困难)。在漏斗口上覆以8层纱布,外
Since 1957, Simon Holdowsky proposed fibrin membrane filtration method for antibiotic sterility testing, the continuous improvement of this method has gradually become perfect [Pharmaceutical inspection work communication 7 (3): 173,1966], but was Chinese Pharmacopoeia 1977 edition a Department of the collection. Film method has a wide range of subjects, bacteria detection of high sensitivity, high activity advantages. However, the shortcoming of this law is the high requirement of aseptic operation, otherwise it is easy to infect bacteria and cause the experiment to fail. Now put forward the points of attention in the operation, for your reference. 1. After the film is clamped in the Chua funnel (hereinafter referred to as funnel), it is moistened with water (to avoid brittleness of the film after high pressure, resulting in difficulty of taking out after suction filtration). In the funnel mouth covered with 8 layers of gauze, outside