论文部分内容阅读
猿猴空泡病毒40(Simian vacuolating virus 40,SV40) 属于乳多空病毒科,是一种DNA肿瘤病毒。亚洲猿类特别是恒河猴是SV40的天然宿主。感染SV40病毒可导致猴体急性病变或呈长期带毒状态,此外能诱使幼鼠产生肿瘤,并能使多种培养细胞发生转化。本研究初步建立了SV40 病毒在Vero细胞中的增殖培养方法,并且初步建立了β丙内脂灭活病毒的方法和纯化工艺。使用SV40病毒灭活疫苗对Balb/c小鼠进行了免疫,结果表明该疫苗具有较好的免疫原性。随后对SV40 病毒DNA在免疫小鼠的重要脏器中的整合情况进行了调查,结果表明SV40病毒DNA未在小鼠重要脏器中整合。本研究为SV40病毒灭活疫苗的研制和进一步开展猴体抗SV40 感染实验奠定了良好的基础。
Simian vacuolating virus 40 (SV40) belongs to the family Papovaviridae and is a DNA tumor virus. Asian apes, especially rhesus monkeys, are the natural hosts for SV40. Infection with SV40 virus can cause acute lesions or long-term status of the monkey, in addition to luring mice to induce tumors, and a variety of cultured cells can be transformed. In this study, we initially established a method of proliferation and culture of SV40 virus in Vero cells, and initially established a method and purification process of β-propiolactone inactivated virus. Balb / c mice were immunized with the SV40 virus inactivated vaccine and the results showed that the vaccine had good immunogenicity. Subsequent investigation of SV40 viral DNA integration in vital organs of immunized mice showed that SV40 viral DNA did not integrate in vital organs of mice. This study laid a good foundation for the development of SV40 virus inactivated vaccine and further experiments on anti-SV40 infection in monkeys.