论文部分内容阅读
目的观察Nbn基因神经特异性敲除小鼠海马齿状回发育的形态学变化,探讨Nbn基因在小鼠海马齿状回发育中的作用。方法采用成熟神经元标记物NeuN,应用免疫组织化学技术结合体视学方法对生后(P)7、14、21天的神经特异性敲除小鼠海马齿状回的发育进行系统观察,并对齿状回各层截面积等参数进行测量,以非基因敲除小鼠为对照组。结果与对照组相比,P7~21天基因敲除小鼠海马齿状回发育明显延缓,分子层、颗粒细胞层及多形层的截面积均减小(P<0.05);颗粒细胞层阳性细胞面数密度也均明显减少(P<0.05);多形层阳性细胞面数密度P21天仍较高(P<0.05)。结论Nbn基因神经特异性敲除小鼠海马齿状回发育明显延缓,提示Nbn基因可能是海马齿状回发育中的重要基因。
Objective To observe the morphological changes of hippocampal dentate gyrus in Nbn gene-specific knockout mice and to explore the role of Nbn gene in the development of dentate gyms in mice. Methods The mature neuronal marker NeuN was used to observe the development of dentate gyrus in hippocampus of neuron-specific knockout mice on postnatal day 7, day 14 and day 21 with immunohistochemistry and stereology. Cross-sectional area of the dentate gyrus and other parameters were measured to non-knockout mice as a control group. Results Compared with the control group, the hippocampal dentate gyrus in P7 ~ 21 days knockout mice was delayed in development and the cross sections of the molecular, granular and pleomorphic layers were decreased (P <0.05); the granular cell layer was positive (P <0.05). The number density of pleomorphic layer positive cells was still high at P21 (P <0.05). Conclusions The degeneration of hippocampal dentate gyrus in Nbn gene-specific knockout mice was significantly delayed, suggesting that Nbn gene may be an important gene in dentate gyrus development.