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目的观察罗格列酮(RGZ)对高糖环境下大鼠肾脏成纤维细胞(NRK)基质金属蛋白酶-1(MMP-1)、基质金属蛋白酶抑制剂-1(TIMP-1)及Ⅲ型胶原(COLⅢ)基因表达的影响,探讨罗格列酮对糖尿病肾病的保护作用及其机制。方法2005年1月至2005年9月对郑州大学第一附属医院的体外培养NRK细胞,分成正常对照组(NG含1000mg/LD-葡萄糖)、高糖组(HG含4500mg/LD-葡萄糖)、HG+RGZ组(5μmol/L)、HG+RGZ组(10μmol/L)和HG+RGZ组(15μmol/L),作用24h后,用逆转录-聚合酶链反应(RT-PCR)的方法检测MMP-1、TIMP-1和collagenⅢmRNA表达。结果HG组细胞MMP-1mRNA水平较NG组下降,差异有显著性(P<0·01),罗格列酮可以一定程度上恢复MMP-1的表达。HG组TIMP-1和COLⅢmRNA水平较NG组升高,差异有显著性(P<0·01),罗格列酮可以剂量依赖性抑制这种高表达。结论罗格列酮可以通过调节MMP-1和TIMP-1的基因表达,抑制高糖导致的肾脏成纤维细胞外基质的蓄积。
Objective To observe the effect of rosiglitazone (RGZ) on the expression of matrix metalloproteinase-1 (MMP-1), matrix metalloproteinase inhibitor-1 (TIMP-1) and type Ⅲ collagen in rat kidney fibroblast (COL Ⅲ) gene expression, to explore the protective effect of rosiglitazone on diabetic nephropathy and its mechanism. Methods From January 2005 to September 2005, NRK cells were cultured in vitro in the First Affiliated Hospital of Zhengzhou University and divided into normal control group (NG containing 1000 mg / L-glucose), high glucose group (HG containing 4500 mg / L-glucose) HG + RGZ group (5μmol / L), HG + RGZ group (10μmol / L) and HG + RGZ group (15μmol / L) for 24 hours, and then detected by reverse transcriptase-polymerase chain reaction MMP-1, TIMP-1 and collagen III mRNA expression. Results The level of MMP-1 mRNA in HG group was significantly lower than that in NG group (P <0.01). Rosiglitazone could restore the expression of MMP-1 to a certain extent. The levels of TIMP-1 and COL Ⅲ mRNA in HG group were significantly higher than those in NG group (P <0.01), and rosiglitazone could inhibit this high-level expression in a dose-dependent manner. Conclusion Rosiglitazone can inhibit the accumulation of extracellular matrix of renal fibroblasts induced by high glucose by regulating the gene expression of MMP-1 and TIMP-1.