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基于水热法合成了形貌均一的纳米Co_3O_4,利用其强催化性能将其连同纳米Pt和辣根过氧化物酶(HRP)层层修饰于金电极表面,最终构建出对H_2O_2具有三重催化作用的新型纳米复合材料Co_3O_4-Pt-HRP修饰电极,并将其应用于对H_2O_2的浓度检测。实验结果发现,纳米Co_3O_4与纳米Pt的催化作用相互协同,并且纳米Pt较大的比表面积和优良的生物相容性增加了后续辣根过氧化物酶的负载量与生物催化活性,从而提高了传感器的灵敏度。在优化条件下,对H_2O_2浓度的检测范围为2.0μmol/L~7.1 mmol/L,检出限为0.9μmol/L。此外,该传感器还表现出较高的选择性、稳定性和灵敏度。
Based on the hydrothermal method, a uniform nano-Co_3O_4 was synthesized and its surface was modified by the strong catalytic properties of nano-Pt and HRP layers. Finally, a three-fold catalytic effect on H_2O_2 was established The novel nanocomposite Co_3O_4-Pt-HRP modified electrode was applied to the determination of H_2O_2. The experimental results showed that the catalytic effect of nano-Co_3O_4 and nano-Pt synergistic, and the larger specific surface area and good biocompatibility of nano-Pt increased the subsequent loading and biocatalytic activity of horseradish peroxidase, thus increasing the Sensitivity of the sensor. Under optimal conditions, the detection limit of H_2O_2 ranged from 2.0 μmol / L to 7.1 mmol / L with a detection limit of 0.9 μmol / L. In addition, the sensor also showed high selectivity, stability and sensitivity.