论文部分内容阅读
目的初步了解肠道病毒(EV)在小儿急性呼吸道感染(ARI)中的流行概况。方法2003-09—2005-04,于首都儿科研究所就诊的815例ARI患儿,取其鼻咽深部分泌物,用病毒分离及逆转录聚合酶链反应(RT-PCR)法检测EV,用病毒分离和间接免疫荧光法检测呼吸道合胞病毒(RSV)等7种呼吸道病毒。取144例患儿双份血清,用酶联免疫吸附试验检测EV抗体。结果病毒总检出率50.9%,RSV阳性率最高(24.9%),其次为EV(16.9%)。EV在不同月份阳性率为0~34.2%,冬季阳性率最低。RT-PCR、双份血抗体检测和病毒分离3种检测EV方法的阳性率分别为16.2%、7.6%和1.0%。332例急性喘息患儿中,EV阳性率20.8%,仅次于RSV(42.5%);3岁以上喘息患儿中,EV阳性率最高(33.9%),而3岁以下患儿中,RSV阳性率最高(48.7%)。结论在住院ARI患儿中,EV是仅次于RSV的常见病原,也是引起小儿急性喘息性疾病的主要病原之一。EV在年长儿中阳性率相对较高,冬季阳性率较低。RT-PCR法检测EV,快速、敏感、特异,可用于小儿EV感染的诊断。
Objective To understand the prevalence of enterovirus (EV) in children with acute respiratory infection (ARI). Methods From September 2003 to April 2005, 815 children with ARI were enrolled in the Capital Institute of Pediatrics and their nasopharyngeal secretions were obtained. EVs were isolated by virus isolation and reverse transcription-polymerase chain reaction (RT-PCR) Virus isolation and indirect immunofluorescence detection of respiratory syncytial virus (RSV) and other 7 kinds of respiratory viruses. Two hundred seventy-four children were enrolled in the study, and EV antibodies were detected by enzyme-linked immunosorbent assay. Results The total positive rate of virus was 50.9%, the positive rate of RSV was the highest (24.9%), followed by EV (16.9%). The positive rate of EV in different months was 0 ~ 34.2%, with the lowest positive rate in winter. The positive rates of RT-PCR, duplex blood antibody detection and virus isolation were 16.2%, 7.6% and 1.0% respectively. Among 332 children with acute wheezing, the positive rate of EV was 20.8%, second only to RSV (42.5%). Among children with wheezing over 3 years old, the positive rate of EV was the highest (33.9%), while among the children under 3 years old, the positive rate of RSV The highest rate (48.7%). Conclusion Among the children with ARI in hospital, EV is the second most common pathogen of RSV and one of the major pathogens causing acute asthmatic disease in children. The positive rate of EV in older children is relatively high, the positive rate in winter is low. RT-PCR detection of EV, fast, sensitive and specific, can be used for pediatric EV infection diagnosis.