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目的:研究4-(4-取代苯胺基)-2-取代苯基-喹唑啉类衍生物[4-(4-substituted aniline)-2-substituted phenyl quinazoline derivatives,Q]对血管紧张素Ⅱ(angiotensin Ⅱ,Ang Ⅱ)诱导的H9C2心肌细胞肥大的影响.方法:体外培养H9C2细胞株,将培养的H9C2心肌细胞随机分为正常组(Con组),Ang Ⅱ组,Ang Ⅱ+Q高剂量(10-6 mol/L)组、Ang Ⅱ+Q中剂量(10-7 mol/L)组、Ang Ⅱ+Q低剂量(10-8 mol/L)组,采用激光共聚焦技术对H9C2细胞的形态扫描成像,利用计算机进行图像处理,对H9C2细胞表面积进行定性定量分析,BCA法测定蛋白浓度,MTT法测定加入不同浓度喹唑啉衍生物后心肌细胞活性,以及通过RT-PCR发测定肥大相关基因mR-NA的表达.结果:与Con组相比,Ang Ⅱ刺激H9C2心肌细胞后,细胞表面积明显增大(P＜0.05);加入喹唑啉衍生物后,H9C2心肌细胞表面积明显减小(P＜0.05).Ang Ⅱ诱导的H9C2心肌细胞,其总蛋白含量和肥大相关基因mRNA的表达水平都明显高于正常的H9C2心肌细胞(P＜0.05),加入喹唑啉衍生物处理后的H9C2心肌细胞其肥大相关基因mRNA的表达明显下降(P＜0.05).结论:4-(4-取代苯胺基)-2-取代苯基-喹唑啉类衍生物可以改善Ang Ⅱ诱导的H9C2心肌细胞肥大.“,”Objective:To define the effects of 4-(4-substituted aniline)-2-substituted phenyl quinazoline derivatives on cardiac hypertrophy.Method:The establishment of cardiomyopathy hypertrophy model through stimulating H9C2 cells with Ang Ⅱ.H9C2 cells were randomized into five groups:normal control group;Ang Ⅱ group;Ang Ⅱ +Q group(10-6 mol/L);Ang Ⅱ +Q group(10-7 mol/L);Ang Ⅱ +Q group(10-8 mol/L).The surface area of H9C2 was measures with a laser scanning confocal microscope technique (LSCM).The total protein content was analyzed by BCA.The survival of cells was assayed by MTT,and the mRNA expressions of hypertrophy-related genes were detected by RT-PCR.Result:Compared with control group,the surface area of H9C2 stimulated by Ang Ⅱ was increased significantly(P＜0.05),after joining Q,the surface area decreased observably (P＜ 0.05).The level of total protein and expressions of genes of hypertrophy of H9C2 stimulated by Ang Ⅱ was increased apparently(P＜0.05),however,those indicators were declined comparatively of H9C2 handled by Q(P＜ 0.05).Conclusion:Quinazoline compound can perfect Ang Ⅱ-induced cardiomyopathy hypertrophy.