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从山东省14个县市区采集的玉米纹枯病标本上分离获得103个玉米纹枯菌菌株。核荧光染色确定菌丝细胞核的数目,以及利用配对培养法确定不同菌株细胞是否融合。结果表明这些菌株分别属于多核丝核菌的AG-1-IA、AG-1-IB、AG-1-IC、AG-3、AG-4-HG-I、AG-5和WAG-Z融合群和双核丝核菌的AG-Ba融合群,其中AG-1-IA类型菌株数量占菌株总数的60.19%,为优势融合群。通过inter-simple sequence repeats(ISSR)标记技术进行菌株的遗传多样性分析,获得45个ISSR分子标记,其中91.1%的片段具有多态性,表明种群间存在丰富的遗传多样性。UPGMA聚类分析将103个菌株分成6个遗传聚类群,遗传聚类群的菌株组成说明遗传群组的划分与菌株的地理来源和菌株融合群类型均存在一定的相关性。
103 strains of WSH were isolated from specimens of corn sheath blight collected from 14 counties and cities in Shandong Province. Nuclear fluorescent staining determines the number of mycelial nuclei and the use of paired culture to determine if different strains of cells fuse. The results showed that these strains belonged to AG-1-IA, AG-1-IB, AG-1-IC, AG-3, AG- 4-HG-I, AG- 5 and WAG-Z fusions And AG-Ba fusion population of Rhizoctonia solani. The number of AG-1-IA type strains accounted for 60.19% of the total number of strains, which was the dominant fusion group. The genetic diversity of the isolates was analyzed by inter-simple sequence repeats (ISSR) markers. 45 ISSR markers were obtained, of which 91.1% were polymorphic, indicating abundant genetic diversity among populations. UPGMA clustering analysis divided 103 strains into 6 genetic clusters, and the genetic composition cluster showed that there was a certain correlation between genetic grouping and geographical origin of strains and fusion types of strains.