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目的 :研究苦参素对肝星状细胞增殖及 型胶原合成的影响 ,阐明其抗肝纤维化的作用机制。方法 :采用袁桃霞 [1 ]等的肝星状细胞的分离方法和 MTT法 (甲基噻唑基四唑 ,m etyl thiazolyl tetronzolium)及 ABC免疫酶染色方法分别检测肝星状细胞增殖及 型胶原合成的影响。结果 :苦参素能明显抑制肝星状细胞的增殖及 型胶原的合成 ,并呈剂量依赖性
Objective: To study the effect of oxymatrine on the proliferation of hepatic stellate cells and the synthesis of collagen, and to elucidate the mechanism of anti-hepatic fibrosis. METHODS: The method of isolating hepatic stellate cells such as Yuan Taoxia [1] and the methods of MTT (methyl thiazolyl tetronzolium) and ABC immunostaining were used to detect the proliferation and type I collagen synthesis of hepatic stellate cells, respectively. influences. RESULTS: Oxymatrine inhibited the proliferation of hepatic stellate cells and the synthesis of collagen in a dose-dependent manner.