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为了探索人血型分型试剂工业化生产的可能性，应用人Ａ、Ｂ血型单克隆抗体，进行了抗体特性及临床血型分型的研究。经血凝特性试验，测得Ｄ２８６－Ｅ１２单克隆抗体仅对Ａ血型特异，６－１－Ｇ１１单克隆抗体仅对Ｂ血型特异。由盐水试管法测得它们的血凝滴度均为１：５１２。以血库常规人抗Ａ、抗Ｂ血清为标准对照，采用盐水试管法，对３７０４例血液样品进行了临床血型分型试验。结果显示，Ａ、Ｂ血型单克隆抗体分型试剂与标准人血清１００％相符。证明：Ｄ２８６－Ｅ１２和６－１－Ｇ１１两株杂交瘤分泌的Ａ、Ｂ血型单克隆抗体可作为非常优越的血型分型试剂。具有工业化生产血型单克隆抗体的潜力。
In order to explore the possibility of industrial production of human blood typing reagents, the application of human monoclonal antibodies A and B to the study of antibody characteristics and clinical blood typing. The hemagglutination properties test, measured D286-E12 monoclonal antibody is only specific to blood type A, 6-1-G11 monoclonal antibody specific only to B blood type. Their blood clot titer was 1: 512 measured by saline test tube method. Blood routine routine anti-A, anti-B serum as a standard control, using saline test tube method, 3704 blood samples for clinical blood typing test. The results showed that A, B blood group monoclonal antibody typing reagent and standard human serum 100% match. It is proved that the monoclonal antibodies of blood group A and B secreted by the two hybridomas of D286-E12 and 6-1-G11 can be regarded as very superior blood typing reagents. Has the potential of industrial production of blood group monoclonal antibody.