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【目的】明确产黄青霉病毒科成员(Botryosphaeria dothidea Chrysovirus 1,BdCV1)对寄主梨轮纹病菌(Botryosphaeria dothidea)菌株生物学性状的影响,证实单独感染BdCV1梨轮纹病菌分离株是弱毒菌株;以期确定BdCV1是否为引起梨轮纹病菌弱致病力的单一因子。【方法】从前期弱致病力复合感染双分体病毒科成员Bd PV1和BdCV1的梨轮纹病菌LW-1中经多代菌丝分离获得的疑似仅单独感染BdCV1分离株(命名为LW-C)为材料;采用dsRNA检测、RTPCR,鉴定LW-C菌株中仅携带BdCV1。采用菌丝块接种于PDA培养基以及3针针刺法接种梨果实,观察其菌落形态以及发病情况,测定菌落生长速度和致病性,明确LW-C生物学特性。将LW-C对无毒菌株Mock进行水平转染,检测其传染后衍生菌株的dsRNA并测定其生物学特性。【结果】证实了LW-C仅感染BdCV1,具有弱毒特性;水平转染结果明确了BdCV1因子对梨轮纹病菌的菌落形态、生长速度有抑制作用,对寄主有弱致病力。【结论】确认了BdCV1是引起梨轮纹病菌致病力衰退的主要因子,为真菌病毒防治果树轮纹病害提供了新颖的生防材料。
【Objective】 The objective of this study was to determine the effect of BdCV1 on the biological characteristics of the host strain Botryosphaeria dothidea. It was confirmed that the isolated BdCV1 strain was weakly virulent. In order to determine whether BdCV1 is a single factor causing the weak pathogenicity of Rhizoctonia cerealis. 【Method】 The isolated BdCV1 isolate (named as LW-1) was isolated from mycorrhizal fungi LW-1 of members of Bd PV1 and BdCV1, C) as material; using dsRNA detection, RTPCR, identification of LW-C strain carrying only BdCV1. The mycelia were inoculated on PDA medium and the 3-acupuncture method was used to inoculate pear fruits. The colony morphology and incidence of colony were observed. The colony growth speed and pathogenicity were determined, and the biological characteristics of LW-C were determined. LW-C was used to transfect non-toxic strain Mock horizontally. The dsRNA of the derivative strain was tested and its biological characteristics were determined. 【Result】 The results showed that LW-C was only infected with BdCV1 and had the characteristics of attenuated virulence. The results of horizontal transfection confirmed that BdCV1 inhibited the colony morphology and growth rate of the pathogen, and had weak virulence to the host. 【Conclusion】 The results showed that BdCV1 was the main factor causing the decline of pathogenicity of pear rot pathogen, and provided a novel biocontrol material for preventing and curing fruit rot disease of tree.