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分别采用Folin-Ciocalteu法测定了水果提取物的总酚含量,采用亚甲基蓝法测定了其抗人肝癌细胞Hep G2和人结肠腺癌细胞Caco-2增殖的活性,分析了总酚含量与抗Hep G2和Caco-2细胞增殖活性之间的相关性。结果显示,25种水果中李子的总酚含量最高(1686.08±96.94μmol GAE/100鲜果),西瓜(83.54±2.10μmol GAE/100 g鲜果)和哈密瓜(79.35±0.76μmol GAE/100 g鲜果)的总酚含量最低;在可测出抗增殖EC50值的水果中,李子(18.99±0.06 mg/m L)和番石榴(20.94±0.09 mg/m L)抗Hep G2细胞增殖的活性最强,梨的活性最弱(389.63±10.82 mg/m L)。李子抗Caco-2细胞增殖的活性最强(8.73±0.11 mg/m L),火龙果的活性最弱(388.07±7.04 mg/m L)。水果的抗Hep G2和Caco-2细胞增殖活性与其总酚含量相关性显著(R2=0.4147,p<0.01;R2=0.4071,p<0.05),该相关性表明水果中的多酚具有良好的抗肿瘤细胞增值的活性。
Folin-Ciocalteu method was used to determine the total phenolic content of the fruit extract, methylene blue method was used to determine its anti-human hepatocellular carcinoma cells Hep G2 and human colon adenocarcinoma cells Caco-2 proliferation activity analysis of total phenolic content and anti Hep G2 And Caco-2 cell proliferation activity. The results showed that the total phenolic content of plum in 25 fruits was highest (1686.08 ± 96.94μmol GAE / 100 fruit), watermelon (83.54 ± 2.10μmol GAE / 100g fruit) and melon (79.35 ± 0.76μmol GAE / 100g fruit) Among the fruits with detectable anti-proliferation EC50 values, plum (18.99 ± 0.06 mg / m L) and guava (20.94 ± 0.09 mg / m L) had the highest proliferation activity in Hep G2 cells. Pear The weakest activity (389.63 ± 10.82 mg / m L). Prunus mongolica had the strongest anti-Caco-2 cell proliferation activity (8.73 ± 0.11 mg / m L) and dragon fruit was the weakest (388.07 ± 7.04 mg / m L). The correlation between the proliferation activity of fruits against Hep G2 and Caco-2 cells was significant (R2 = 0.4147, p <0.01; R2 = 0.4071, p <0.05). The correlation indicated that the polyphenols in fruit had good anti Tumor cell proliferation activity.