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In order to explore whether the conventional use of 5 fluorouracil (5 Fu) had any toxic effects on trabecular meshwork cells, bovine trabecular meshwork cells were cultured in vitro and exposed to 5 Fu at different concentrations. The cellular morphology, ultrastructure, mortality and phagocytosis were studied under light microscopy, transmission electron microscopy and methods of Wright’s stain. It was found that the toxic effects of 5 Fu on the cells were in a dose dependent mode. 1×10 -1 mg/ml of 5 Fu caused a large part of cells rounded up, while 1×10 -3 mg/ml of the drug only a rough appearance of the cell surface. Exposure to 1×10 -2 mg/ml of 5 Fu made mitochrone swollen and rough endoplasmic reticulum enlarged, with the cell mortality being 50.5 %. The latex microspheres engulfed in cytoplasm in cells receiving 1×10 -1 and 1×10 -2 mg/ml of 5 Fu were significantly decreased as compared with those in the control group ( P <0.01). It was concluded that the safe concentration of 5 Fu on bovine trabecular meshwork cells was 1×10 -3 mg/ml and the conventional dosage of 5 Fu in clinical practice would not cause injury to trabecular meshwork cells.
In order to explore whether the conventional use of 5 fluorouracil (5 Fu) had any toxic effects on trabecular meshwork cells, bovine trabecular meshwork cells were cultured in vitro and exposed to 5 Fu at different concentrations. The cellular morphology, ultrastructure, mortality and phagocytosis were studied under light microscopy, transmission electron microscopy and methods of Wright’s stain. It was found that the toxic effects of 5 Fu on the cells were in a dose dependent mode. 1 × 10 -1 mg / ml of 5 Fu caused a large part of cells rounded up, while 1 × 10 -3 mg / ml of the drug only a rough appearance of the cell surface. Exposure to 1 × 10 -2 mg / ml of 5 Fu made mitochrone swollen and rough endoplasmic reticulum enlarged, with the Cell mortality was 50.5%. The latex microspheres engulfed in cytoplasm in cells receiving 1 × 10 -1 and 1 × 10 -2 mg / ml of 5 Fu were significantly decreased as compared with those in the control group (P <0.01). It was conc luded that the safe concentration of 5 Fu on bovine trabecular meshwork cells was 1 × 10 -3 mg / ml and the conventional dosage of 5 Fu in clinical practice would not cause injury to trabecular meshwork cells.