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近20多年来,随着穿透性角膜移植术的开展,各种角膜保存法应运而生,并在世界范围内得到不同程度的使用。最初,人们将摘除下来的整个眼球放入湿房4℃保存。这种方法简单易行,但最多只能保存角膜48小时。确切地说,这并不是一种真正的角膜保存法,而只是一种眼球贮存法。1965年,Capella、Kaufman和Robbins发明了角膜冷冻保存法,这使角膜组织的长期保存首次得以实现。虽然它解决了一些角膜保存方面的重要问题,但因操作步骤复杂,需要高级技术人员和昂贵的仪器设施,故实际上仅在一些大的研究机构获得了成功。Lindstrom
In the past 20 years, with the development of penetrating keratoplasty, various corneal preservation methods came into being, and have been used to varying degrees in the world. Initially, people will be removed from the entire eye into the wet room 4 ℃ save. This method is simple, but can save the cornea up to 48 hours. Specifically, this is not a true corneal preservation method, but an eyeball storage method. In 1965, Capella, Kaufman, and Robbins invented the corneal cryopreservation method, making the first long-term preservation of corneal tissue possible. Although it addresses some of the important issues of corneal preservation, it has in fact been successful only in some of the largest research institutes because of the complex steps involved, the need for senior technicians and expensive equipment. Lindstrom