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Mesenchymal stem cell(MSC)-mediated therapy has been shown to be clinically effective in regenerating tissue defects. For improved regenerative therapy, it is critical to isolate homogenous populations of MSCs with high capacity to differentiate into appropriate tissues. The utilization of stem cell surface antigens provides a means to identify MSCs from various tissues. However, few surface markers that consistently isolate highly regenerative MSCs have been validated, making it challenging for routine clinical applications and making it all the more imperative to identify reliable surface markers. In this study, we used three surface marker combinations:CD51/CD140 a, CD271, and STRO-1/CD146 for the isolation of homogenous populations of dental mesenchymal stem cells(DMSCs)from heterogeneous periodontal ligament cells(PDLCs). Fluorescence-activated cell sorting analysis revealed that 24% of PDLCs were CD51~+/CD140a~+, 0.8% were CD271~+, and 2.4% were STRO-1~+/CD146~+. Sorted cell populations were further assessed for their multipotent properties by inducing osteogenic and chondrogenic differentiation. All three subsets of isolated DMSCs exhibited differentiation capacity into osteogenic and chondrogenic lineages but with varying degrees. CD271~+ DMSCs demonstrated the greatest osteogenic potential with strong induction of osteogenic markers such as DLX5, RUNX2, and BGLAP. Our study provides evidence that surface marker combinations used in this study are sufficient markers for the isolation of DMSCs from PDLCs. These results provide important insight into using specific surface markers for identifying homogenous populations of DMSCs for their improved utilization in regenerative medicine.
Mesenchymal stem cell (MSC) -mediated therapy has been shown to be clinically effective in regenerating tissue defects. It is critical to isolate regenerative tissue defects. It is critical to isolate the individual populations of MSCs with high capacity to differentiate into appropriate tissues. The utilization of stem cell surface Antigens provides a means to identify MSCs from various tissues. However, few surface markers that consistently isolate highly regenerative MSCs have been validated, making it challenging for routine clinical applications and making it all the more imperative to identify reliable surface markers. In this study, Fluorescence-activated cell sorting analysis (CDLC): CD51 / CD140 a, CD271, and STRO-1 / CD146 for the isolation of homogenous populations of dental mesenchymal stem cells (DMSCs) from heterogeneous periodontal ligament cells that 24% of PDLCs were CD51 ~ + / CD140a ~ +, 0.8% were CD271 ~ +, and 2.4% were STRO-1 ~ + / CD146 ~ +. all three subsets of isolated osteogenic and chondrogenic differentiation. as DLX5, RUNX2, and BGLAP. Our study provides evidence that surface marker combinations used in this study are sufficient markers for the isolation of DMSCs from PDLCs. These results provide important insight into using specific surface markers for identifying homogenous populations of DMSCs for their improved utilization in regenerative medicine.