The isolation and purification of cardiac mitochondria by differential and iodixanol density gradien

• 出 处: 中国病理生理杂志
• 发表日期: 2012年11期
• 关键词: mitochondria isolation purification purified trophy mitochondrial discontinuous

To obtain the function of complete myocardial mitochondria,the isolation procedures started with differential centrifugation of the heart homogenate.Mitochondria were purified by using iodixanol in three different ways:(1)continuous gradients 19%to 27%

To obtain the function of complete myocardial mitochondria, the isolation procedures started with differential centrifugation of the heart homogenate. Mitochondria were purified by using iodixanol in three different ways: (1) continuous gradients 19% to 27%