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目的检测和分析性病门诊女性患者宫颈分泌物中人乳头瘤病毒(HPV)的基因型别。方法收集我院性病门诊就诊的女性患者378例,按患者有无高危接触和宫颈口有无尖锐湿疣症状划分为3组,即有接触有症状组(161例)、有接触无症状组(117例)和无接触无症状组(100例)。取3组患者脱落细胞,采用反向分子杂交法进行HPV基因分型检测。结果性病门诊378例女性宫颈分泌物HPV阳性率为56.61%(214/378),有接触有症状组、有接触无症状组和无接触无症状组的阳性率分别为98.75%(159/161)、40.17%(47/117)和8.00%(8/100),3组分别检出22种、14种和5种HPV基因型别,检出的主要型别是HPV6、11、16、58、56、18、33、68。有接触有症状组和有接触无症状组单一感染和复合感染的感染率差异无统计学意义(P>0.05),在多重复合感染中,以二重和三重感染为主,最多发现八重感染。有接触有症状组低危型121例次(76.10%),高危型99例次(62.26%),有接触无症状组低危型35人次(74.46%),高危型26例次(55.32%),两组感染率较高,但差异无统计学意义(P>0.05)。结论 HPV6、11、16、58、56、18、33、68是性病门诊女性患者宫颈感染HPV的主要型别,健康体检者中亦能查出,临床应加强对性病门诊女性就诊者宫颈HPV基因的检测,及对HPV阳性患者进行定期的跟踪随访,尤其是高危型和复合感染患者。
Objective To detect and analyze genotypes of human papillomavirus (HPV) in cervical secretions of sexually transmitted disease outpatients. Methods A total of 378 female patients admitted to our STD clinics were divided into 3 groups according to the presence or absence of high-risk patients and the presence or absence of condyloma acuminatum in the cervix. There were 161 symptomatic patients and 117 asymptomatic patients Cases) and no contact asymptomatic group (100 cases). Take 3 groups of patients exfoliated cells, using reverse molecular hybridization HPV genotyping test. Results The positive rate of HPV positive rate of cervical secretions in 378 outpatient clinics was 56.61% (214/378). The positive rates of HPV with contact symptomatic group, asymptomatic contact group and non-contact asymptomatic group were 98.75% (159/161) , 40.17% (47/117) and 8.00% (8/100) respectively. Twenty-two, 14 and five HPV genotypes were detected in the three groups. The major types detected were HPV6, 11, 16, 58, 56,18,33,68. There was no significant difference in the infection rates of single infection and multiple infection between symptomatic and asymptomatic groups (P> 0.05). Duplex and triple infections were the most common infection in multiple complication infection, and the most frequent infection was ocular infection. There were 121 cases (76.10%) with low risk type and 99 cases (62.26%) with high risk type, 35 low risk type (74.46%) with asymptomatic symptoms and 26 cases (55.32%) with high risk type, , But the difference was not statistically significant (P> 0.05). Conclusion HPV6,11,16,58,56,18,33,68 is the main type of cervical HPV infection in STD clinics, which can be detected in healthy subjects. Clinics should strengthen the diagnosis of cervical HPV gene , And regular follow-up of HPV-positive patients, especially in high-risk and co-infected patients.