【摘 要】
：
运用Fe2 + Vc损伤体系诱导大鼠肝线粒体氧化损伤 ,并研究了灯盏乙素的抑制作用 .采用分光光度法测定脂质过氧化物丙二醛 (MDA)含量、膜蛋白巯基含量及线粒体肿胀度 ,利用荧
【机 构】
：
华中科技大学药物研究所,华中科技大学药物研究所,华中科技大学药物研究所,华中科技大学药物研究所
论文部分内容阅读
运用Fe2 + Vc损伤体系诱导大鼠肝线粒体氧化损伤 ,并研究了灯盏乙素的抑制作用 .采用分光光度法测定脂质过氧化物丙二醛 (MDA)含量、膜蛋白巯基含量及线粒体肿胀度 ,利用荧光染料Rhodamine12 3对线粒体进行标记 ,跟踪线粒体膜电位的变化 .研究发现 2 0～ 5 0 μmol/L的灯盏乙素能抑制MDA的生成并促使膜巯基含量下降 ,对线粒体肿胀和膜电位的降低都有一定的抑制作用 ,并呈剂量依赖关系 .结果表明灯盏乙素是一种有效的抗氧化剂 ,能抑制活性氧诱导的线粒体氧化损伤
The oxidative damage of rat liver mitochondria was induced by Fe2 + Vc injury system, and the inhibitory effect of scutellarin was studied. The content of lipid peroxide malondialdehyde (MDA), membrane protein thiol content and mitochondrial swelling degree were measured by spectrophotometry. Fluorescence dye Rhodamine12 3 was used to label mitochondria to track the changes of mitochondrial membrane potential. It was found that 20-50 μmol/L scutellarin could inhibit the production of MDA and promote the decrease of membrane thiol content, and mitochondrial swelling and membrane potential. The reduction has a certain inhibitory effect and is dose-dependent. The results show that scutellarin is an effective antioxidant and can inhibit ROS-induced mitochondrial oxidative damage.
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